Effects of Interferon Gamma on the Antibody Response to Pseudomonas aeruginosa Lipopolysaccharide in Mice
| Autor: | Philip W. Stashak, Tomas Hraba, Christopher E. Taylor, Michael B. Fauntleroy, P J Baker |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Lipopolysaccharides
Lipopolysaccharide Immunology Congenic chemical and pharmacologic phenomena Biology medicine.disease_cause Major histocompatibility complex Major Histocompatibility Complex Interferon-gamma Mice Mice Inbred AKR chemistry.chemical_compound medicine Animals Immunology and Allergy Interferon gamma Mice Inbred BALB C Mice Inbred C3H Pseudomonas aeruginosa Antibodies Monoclonal Hematology Antibodies Bacterial Molecular biology Isotype Immunoglobulin Isotypes chemistry Immunization Mice Inbred DBA biology.protein Female Antibody medicine.drug |
| Zdroj: | Immunobiology. 192:106-120 |
| ISSN: | 0171-2985 |
| DOI: | 10.1016/s0171-2985(11)80411-0 |
| Popis: | Different strains of mice were examined for the capacity to produce an Ig subclass-specific antibody response to purified Pseudomonas aeruginosa lipopolysaccharide (PALPS). With the exception of the AKR strain, the predominant isotype for most of the strains tested was IgG3 whereas the least frequent isotype expressed was either IgG2b or IgGl. AKR mice were unique in that the predominant isotype produced was IgG2a, rather than IgG3; however, the administration of anti-interferon gamma antibody, at the time of immunization with PALPS caused a substantial decrease in the IgG2a antibody response. Selected B10 congenic strains were used to assess the relationship between the antibody responses and the major histocompatibility complex (MHC) genes. Here, the isotype-patterns for the antibody responses were essentially the same regardless of the MHC haplotype. Interestingly, an increase in IgG2a, with a concomitant decrease in IgM and IgG1 antibody was noted when C3H mice were given interferon gamma at the time of immunization. These studies indicate that, in general, the antibody response to PALPS consists of IgG3 antibody as the predominant isotype, and that the antibody response can be modified by interferon gamma. |
| Databáze: | OpenAIRE |
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