Prion infection in cells is abolished by a mutated manganese transporter but shows no relation to zinc
| Autor: | James P. Barnett, David R. Brown, Karen Frudd, Claudia A. Blindauer, Rachel Pass |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Prions
animal diseases chemistry.chemical_element Manganese Zinc Calcium-Transporting ATPases Transfection DNA-binding protein Cell Line Cellular and Molecular Neuroscience Mice Metalloprotein Animals Receptors AMPA Molecular Biology Cation Transport Proteins chemistry.chemical_classification biology Membrane transport protein Membrane Transport Proteins Transporter Cell Biology Transport protein nervous system diseases DNA-Binding Proteins chemistry Biochemistry Cell culture Mutation biology.protein Endopeptidase K Transcription Factors |
| Zdroj: | Pass, R, Frudd, K, Barnett, J P, Blindauer, C A & Brown, D R 2015, ' Prion infection in cells is abolished by a mutated manganese transporter but shows no relation to zinc ', Molecular and Cellular Neuroscience, vol. 68, pp. 186-193 . https://doi.org/10.1016/j.mcn.2015.08.004 |
| DOI: | 10.1016/j.mcn.2015.08.004 |
| Popis: | The cellular prion protein has been identified as a metalloprotein that binds copper. There have been some suggestions that prion protein also influences zinc and manganese homeostasis. In this study we used a series of cell lines to study the levels of zinc and manganese under different conditions. We overexpressed either the prion protein or known transporters for zinc and manganese to determine relations between the prion protein and both manganese and zinc homeostasis. Our observations supported neither a link between the prion protein and zinc metabolism nor any effect of altered zinc levels on prion protein expression or cellular infection with prions. In contrast we found that a gain of function mutant of a manganese transporter caused reduction of manganese levels in prion infected cells, loss of observable PrP(Sc) in cells and resistance to prion infection. These studies strengthen the link between manganese and prion disease. |
| Databáze: | OpenAIRE |
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