Recognition of Histone Crotonylation by Taf14 Links Metabolic State to Gene Expression
| Autor: | Ashby J. Morrison, Raghuvar Dronamraju, Stephen A. Shinsky, Graeme J. Gowans, Natarajan V. Bhanu, Nicolas E. Buchler, Joseph B. Bridgers, Aline Thiengmany, Anthony Burnetti, Devin A. King, Jibo Zhang, Brian D. Strahl, Benjamin A. Garcia |
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| Rok vydání: | 2019 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Transcription Genetic Saccharomyces cerevisiae Article Histones 03 medical and health sciences 0302 clinical medicine Transcription (biology) Gene Expression Regulation Fungal Gene expression Homeostasis Molecular Biology Psychological repression Gene 030304 developmental biology Regulation of gene expression 0303 health sciences biology Lysine Fatty Acids Cell Biology Chromatin Cell biology Histone biology.protein Transcription Factor TFIID Acyl Coenzyme A Signal transduction Energy Metabolism Oxidation-Reduction Protein Processing Post-Translational 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Mol Cell |
| ISSN: | 1097-2765 |
| Popis: | Metabolic signaling to chromatin often underlies how adaptive transcriptional responses are controlled. While intermediary metabolites serve as co-factors for histone-modifying enzymes during metabolic flux, how these modifications contribute to transcriptional responses is poorly understood. Here, we utilize the highly synchronized yeast metabolic cycle (YMC) and find that fatty acid β-oxidation genes are periodically expressed coincident with the β-oxidation byproduct histone crotonylation. Specifically, we found that H3K9 crotonylation peaks when H3K9 acetylation declines and energy resources become limited. During this metabolic state, pro-growth gene expression is dampened; however, mutation of the Taf14 YEATS domain, a H3K9 crotonylation reader, results in de-repression of these genes. Conversely, exogenous addition of crotonic acid results in increased histone crotonylation, constitutive repression of pro-growth genes, and disrupted YMC oscillations. Together, our findings expose an unexpected link between metabolic flux and transcription, and demonstrate that histone crotonylation and Taf14 participate in the repression of energy-demanding gene expression. |
| Databáze: | OpenAIRE |
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