CD4+CD25+ regulatory T cells in the small intestinal lamina propria show an effector/memory phenotype
| Autor: | Takako Hirata, Zhongbin Bai, Mika Nishiyama, Masanori Matsumoto, Kouji Matsushima, Eiji Umemoto, Mikako Yamasaki, Masayuki Miyasaka, Kazuhiro Otani, Zijin Guo, Satoshi Ueha, Myoung Ho Jang |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
CD4-Positive T-Lymphocytes
Chemokine Immunology chemical and pharmacologic phenomena C-C chemokine receptor type 7 T-Lymphocytes Regulatory Mice Antigens CD medicine Animals Immunology and Allergy CCL17 CTLA-4 Antigen IL-2 receptor Intestinal Mucosa CXCL16 Chemokine CCL22 Mice Inbred BALB C Lamina propria biology Interleukin-2 Receptor alpha Subunit Forkhead Transcription Factors hemic and immune systems Dendritic Cells General Medicine Molecular biology Phenotype medicine.anatomical_structure Gene Expression Regulation biology.protein XCL2 Receptors Chemokine Chemokine CCL17 Lymph Nodes CCL25 Immunologic Memory |
| Zdroj: | International Immunology. 20:307-315 |
| ISSN: | 1460-2377 0953-8178 |
| DOI: | 10.1093/intimm/dxm143 |
| Popis: | CD4(+)CD25(+) regulatory T cells (Tregs) have been implicated in the suppression of pathogenic responses to both self- and non-self-antigens in the intestine. However, their precise properties and functions in the gut, as well as the molecular basis of their recruitment to the gut, are poorly understood. Here, we found that most of the CD4(+)CD25(+) T cells in the small intestinal lamina propria (LP) express Foxp3 and exhibit an 'effector/memory' phenotype, CD44(hi)CD45RB(lo)CD62L(-), whereas only a minority of the Foxp3(+)CD4(+)CD25(+) T cells in the spleen and mesenteric lymph nodes showed this phenotype. The Tregs in the small intestinal LP (LP-Tregs) expressed higher levels of CCR4 and CCR9 and a substantially lower level of CCR7 than the Tregs in the spleen. In vitro, the LP-Tregs showed chemotaxis to CCL25/thymus-expressed chemokine. In addition, they showed efficient chemotaxis to the CCR4 ligands, CCL17/thymus and activation-regulated chemokine and CCL22/macrophage-derived chemokine, which are abundantly expressed by dendritic cells (DCs) in the small intestinal LP. In vivo, approximately 50% of the LP-Tregs were closely associated or in direct contact with LP-DCs. These findings demonstrate that LP-Tregs are phenotypically and functionally unique and raise the possibility that they are retained in the small intestinal LP through the action of CCL17 and CCL22, which are locally produced by LP-DCs. |
| Databáze: | OpenAIRE |
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