Effects of basic fibroblast growth factors on hypertrophic scarring in a rabbit ear model
| Autor: | Bing Huang, Tian-zeng Li, Julin Xie, Bin Shu, Xusheng Liu, Jingming Tang, Hui-zhen Liang, Ying-bin Xu, Shao-hai Qi |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Chronic wound
Pathology medicine.medical_specialty Integrins Cicatrix Hypertrophic Administration Topical Basic fibroblast growth factor Enzyme-Linked Immunosorbent Assay Dermatology Fibroblast growth factor Muscle hypertrophy chemistry.chemical_compound medicine Animals Ear External Wound Healing business.industry Reverse Transcriptase Polymerase Chain Reaction Blotting Northern Surgery chemistry Hypertrophic scarring Collagen metabolism Fibroblast Growth Factor 2 Collagen Rabbits medicine.symptom Matrix Metalloproteinase 1 Wound healing business |
| Zdroj: | Journal of cutaneous medicine and surgery. 12(4) |
| ISSN: | 1203-4754 |
| Popis: | Background: Basic fibroblast growth factor (bFGF) was clinically proven to accelerate acute and chronic wound healing. Accelerated wound healing may lead to improved scarring. These studies suggested a possible antiscarring effect of bFGF during wound healing. Little was known about the precise pathologic mechanisms of bFGF on scarring formation. Aims: The aim of this study was to investigate the effect of bFGF on hypertrophic scarring in a rabbit ear model and clarify the mechanism of bFGF on scar treatment. Methods: The rabbit model of hypertrophic scarring was created and received of a low- or high-dose topical treatment three times daily for 1, 2, or 3 months. Then we examined the changes in the macroscopic and histopathologic characteristics of the scars. The expression of collagen, α1β2 integrin, and matrix metalloproteinase 1 (MMP-1) was studied by applying reverse transcriptase-polymerase chain reaction, enzyme-linked immunosorbent assay, and Western blotting. Result: High-dose bFGF remarkably alleviated the scar in the rabbit ear model and decreased collagen type I expression. Further study revealed that bFGF remarkably enhanced MMP-1 and decreased α1β2 integrin expression. Conclusion: This study supports the hypothesis that bFGF exerted a net negative effect on collagen remodeling, therefore suggesting a potential antiscarring role. |
| Databáze: | OpenAIRE |
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