Human platelet-rich plasma- and extracellular matrix-derived peptides promote impaired cutaneous wound healing in vivo
| Autor: | Jeffry M R Deckenback, Lindsey Wolf, Michael R. Hamblin, Tatiana N. Demidova-Rice, Ira M. Herman |
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| Přispěvatelé: | Harvard University--MIT Division of Health Sciences and Technology, Hamblin, Michael R. |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Keratinocytes
Proteomics Pathology Angiogenesis Fibrillin-1 lcsh:Medicine Biochemistry Extracellular matrix Cell Movement Nucleic Acids Molecular Cell Biology Morphogenesis lcsh:Science Cells Cultured Skin Protein Synthesis Inhibitors Multidisciplinary biology Neovascularization Pathologic integumentary system Chemistry Platelet-Rich Plasma Microfilament Proteins Granulation tissue Tenascin Animal Models Chromatography Ion Exchange Cell biology Extracellular Matrix medicine.anatomical_structure Chromatography Gel Medicine Cellular Types Research Article Blood Platelets medicine.medical_specialty Drugs and Devices Histology Biophysics Dermatology Epithelial cell migration Fibrillins Model Organisms In vivo medicine Animals Humans Cyclophosphamide Biology Cell Proliferation Wound Healing lcsh:R Endothelial Cells Platelet-rich plasma biology.protein Cattle lcsh:Q Wound healing Peptides Developmental Biology |
| Zdroj: | PLoS ONE, Vol 7, Iss 2, p e32146 (2012) PLoS ONE PLoS |
| ISSN: | 1932-6203 |
| Popis: | Previous work in our laboratory has described several pro-angiogenic short peptides derived from endothelial extracellular matrices degraded by bacterial collagenase. Here we tested whether these peptides could stimulate wound healing in vivo. Our experiments demonstrated that a peptide created as combination of fragments of tenascin X and fibrillin 1 (comb1) applied into cranial dermal wounds created in mice treated with cyclophosphamide to impair wound healing, can improve the rate of wound closure. Furthermore, we identify and characterize a novel peptide (UN3) created and modified from two naturally-occurring peptides, which are present in human platelet-rich plasma. In vitro testing of UN3 demonstrates that it causes a 50% increase in endothelial proliferation, 250% increase in angiogenic response and a tripling of epithelial cell migration in response to injury. Results of in vivo experiments where comb1 and UN3 peptides were added together to cranial wounds in cyclophosphamide-treated mice leads to improvement of wound vascularization as shown by an increase of the number of blood vessels present in the wound beds. Application of the peptides markedly promotes cellular responses to injury and essentially restores wound healing dynamics to those of normal, acute wounds in the absence of cyclophosphamide impairment. Our current work is aimed at understanding the mechanisms underlying the stimulatory effects of these peptides as well as identification of the cellular receptors mediating these effects. National Institutes of Health (U.S.) (Grant EY15125) National Institutes of Health (U.S.) (Grant EY19533) Wound Care Partners, LLC |
| Databáze: | OpenAIRE |
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