Rubus imperialis (Rosaceae) extract and pure compound niga-ichigoside F1: wound healing and anti-inflammatory effects

Autor: Maria Luisa de Oliveira Nunes, Marcel Petreanu, Liliani Carolini Thiesen, Marina Jagielski Goss, Marcos Paulo Donato, Milena Fronza Broering, José Roberto Santin, Rivaldo Niero, Isabel Daufenback Machado, Talita Dacroce Tonin
Rok vydání: 2016
Předmět:
0301 basic medicine
Lipopolysaccharides
Male
Leukocyte migration
Antioxidant
DPPH
Neutrophils
medicine.medical_treatment
Anti-Inflammatory Agents
Wounds
Penetrating
Pharmacology
Carrageenan
Antioxidants
chemistry.chemical_compound
0302 clinical medicine
Skin
Mice
Inbred BALB C
General Medicine
Chemotaxis
Leukocyte
Neutrophil Infiltration
030220 oncology & carcinogenesis
medicine.drug_class
Biology
Anti-inflammatory
Cell Line
03 medical and health sciences
Picrates
In vivo
Botany
medicine
Animals
Efferocytosis
Inflammation
Wound Healing
Plants
Medicinal
Dose-Response Relationship
Drug
Plant Extracts
Methanol
Biphenyl Compounds
Hydrogen Peroxide
Plant Components
Aerial
Saponins
Disease Models
Animal
Oxidative Stress
030104 developmental biology
chemistry
Macrophages
Peritoneal
Solvents
Wound healing
Rubus
Phytotherapy
Zdroj: Naunyn-Schmiedeberg's archives of pharmacology. 389(11)
ISSN: 1432-1912
Popis: Here, we evaluate the anti-inflammatory and wound-healing effects of methanolic crude extract obtained from aerial parts (leaves and branches) of Rubus imperialis Chum. Schl. (Rosaceae) and the pure compound niga-ichigoside F1. Anti-inflammatory activity was determined in vivo and in vitro, and the healing effect was evaluated in surgical lesions in mice skin. The 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) assay and H2O2-induced oxidative stress were used to determine antioxidant activity. The efferocytosis activity was also determined. The data obtained show that the extract of R. imperialis promote reduction in the inflammatory process induced by lipopolysaccharide (LPS) or carrageenan in the air pouch model; the effects could be reinforced by nitric oxide reduction in LPS-stimulated neutrophils, and an increase in the efferocytosis. The extract showed wound healing property in vitro and in vivo, scavenging activity for DPPH, and cytoprotection in the H2O2-induced oxidative stress in L929 cells. In addition, the compound niga-ichigoside F1 was able to reduce the NO secretion; however, it did not present wound-healing activity in vitro. Together, the data obtained point out the modulatory actions of R. imperialis extract on leukocyte migration to the inflamed tissue, the antioxidant, and the pro-resolutive activity. However, the R. imperialis anti-inflammatory activity may be mediated in parts by niga-ichigoside F1, and on wound healing do not correlated with niga-ichigoside F1.
Databáze: OpenAIRE
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