Adaptation of motor unit contractile properties in rat medial gastrocnemius to treadmill endurance training: Relationship to muscle mitochondrial biogenesis
| Autor: | H. Drzymala-Celichowska, Jan Celichowski, Anna Gawędzka, Michal Korostynski, Elżbieta Pyza, Joanna Majerczak, Katarzyna Kryściak, Jakub Kryściak, Jerzy A. Zoladz, Zbigniew Szkutnik, Magdalena Guzik, Dominik J. Kaczmarek, Dawid Łochyński, Wieslawa Jarmuszkiewicz, Piotr Krutki |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Bacterial Diseases Male MFN2 Muscle Proteins Mitochondrion Pathology and Laboratory Medicine Biochemistry GTP Phosphohydrolases Running Random Allocation 0302 clinical medicine Medicine and Health Sciences MFN1 Energy-Producing Organelles Fatigue Multidisciplinary Organelle Biogenesis Adaptation Physiological Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha Mitochondrial DNA Mitochondria Nucleic acids Bioassays and Physiological Analysis Infectious Diseases Muscle Fatigue Medicine Cellular Structures and Organelles Muscle Electrophysiology Research Article Muscle Contraction medicine.medical_specialty Forms of DNA Science Motor Proteins Biology Bioenergetics Biosynthesis Research and Analysis Methods DNA Mitochondrial Mitochondrial Proteins 03 medical and health sciences Signs and Symptoms Endurance training Diagnostic Medicine Molecular Motors Internal medicine Physical Conditioning Animal medicine Genetics Animals RNA Messenger Rats Wistar Muscle Skeletal Tetanus Electrophysiological Techniques Biology and Life Sciences Proteins Membrane Proteins Cell Biology DNA Motor unit 030104 developmental biology Endocrinology Mitochondrial biogenesis Electron Transport Chain Complex Proteins Gene Expression Regulation Exercise Test Physical Endurance Organelle biogenesis 030217 neurology & neurosurgery |
| Zdroj: | PLoS ONE, Vol 13, Iss 4, p e0195704 (2018) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | This study aimed at investigating the effects of 2, 4 and 8 weeks of endurance training on the contractile properties of slow (S), fast fatigue resistant (FR) and fast fatigable (FF) motor units (MUs) in rat medial gastrocnemius (MG) in relation to the changes in muscle mitochondrial biogenesis. The properties of functionally isolated MUs were examined in vivo. Mitochondrial biogenesis was judged based on the changes in mitochondrial DNA copy number (mtDNA), the content of the electron transport chain (ETC) proteins and PGC-1α in the MG. Moreover, the markers of mitochondria remodeling mitofusins (Mfn1, Mfn2) and dynamin-like protein (Opa1) were studied using qPCR. A proportion of FR MUs increased from 37.9% to 50.8% and a proportion of FF units decreased from 44.7% to 26.6% after 8 weeks of training. The increased fatigue resistance, shortened twitch duration, and increased ability to potentiate force were found as early as after 2 weeks of endurance training, predominantly in FR MUs. Moreover, just after 2 weeks of the training an enhancement of the mitochondrial network remodeling was present as judged by an increase in expression of Mfn1, Opa1 and an increase in PGC-1α in the slow part of MG. Interestingly, no signs of intensification of mitochondrial biogenesis assessed by ETC proteins content and mtDNA in slow and fast parts of gastrocnemius were found at this stage of the training. Nevertheless, after 8 weeks of training an increase in the ETC protein content was observed, but mainly in the slow part of gastrocnemius. Concluding, the functional changes in MUs' contractile properties leading to the enhancement of muscle performance accompanied by an activation of signalling that controls the muscle mitochondrial network reorganisation and mitochondrial biogenesis belong to an early muscle adaptive responses that precede an increase in mitochondrial ETC protein content. |
| Databáze: | OpenAIRE |
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