Routine cytogenetic prenatal diagnosis using dynamic banding (RBG-GBG): a highly reproducible method for amniocytes, fetal cord blood, and chorionic villus investigations
| Autor: | F. Vasseur, A. Daudignon, Savary Jb, Laï Jl, Deminatti Mm |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
medicine.medical_specialty
Pathology Chorionic villus sampling Prenatal diagnosis Chromosome Disorders Biology S Phase Pregnancy Prenatal Diagnosis medicine Humans Diagnosis Computer-Assisted Genetics (clinical) Chromosome Aberrations medicine.diagnostic_test Cytogenetics Obstetrics and Gynecology Karyotype Fetal Blood Staining Chromosome Banding medicine.anatomical_structure Bromodeoxyuridine Chorionic Villi Sampling Karyotyping Amniocentesis Chorionic villi Female Nucleolus organizer region |
| Zdroj: | Prenatal diagnosis. 11(12) |
| ISSN: | 0197-3851 |
| Popis: | Dynamic banding (RBG-GBG) using pulse 5-bromodeoxyuridine (5-BrdU) incorporation during part of the last S-phase before harvesting has been used in prenatal investigations. This method has already been routinely applied in 1344 cytogenetic investigations. GBG and RBG bandings produced almost identical patterns to classical G- and R-banding methods except for heterochromatic portions and some euchromatic segments. Nevertheless, these discordances may be somewhat helpful for cytogenetic diagnosis (i.e., X numerical abnormalities). The results showed particularly good contrast and staining; 5-BrdU incorporation did not prevent additional staining. Likewise, previous RBG or GBG disclosure allowed further chromosomal identification with C-banding or nucleolar organizer staining. Simplicity and reproducibility were very helpful in cases with a low mitotic index. 5-BrdU had no significant effect on in-vitro damage because only 0.31 percent of cells were affected; so, we believe that dynamic banding should be used more extensively in cytogenetic investigations. Moreover, the staining and contrast qualities were very suitable for automatic methods of analysis now in use: i.e., metaphase finding and computer-assisted karyogram creation. |
| Databáze: | OpenAIRE |
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