Development of ultra-high sensitivity bioluminescent enzyme immunoassay for prostate-specific antigen (PSA) using firefly luciferase
| Autor: | Toshihiko Iba, Katsushi Abe, Yoshiaki Seto |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Luminescence
Biophysics urologic and male genital diseases Sensitivity and Specificity Management of prostate cancer Immunoenzyme Techniques Antigen medicine Animals Humans Luciferase Luciferases Detection limit Chromatography biology medicine.diagnostic_test Chemistry Immunomagnetic Separation Antibodies Monoclonal Prostate-Specific Antigen Reference Standards Molecular biology Coleoptera Prostate-specific antigen Chemistry (miscellaneous) Biotinylation Immunoassay biology.protein Female Antibody |
| Zdroj: | Luminescence : the journal of biological and chemical luminescence. 16(4) |
| ISSN: | 1522-7235 |
| Popis: | A bioluminescent enzyme immunoassay (BLEIA) for prostate-specific antigen (PSA) using biotinylated firefly luciferase-labelled antibody was developed. PSA is an important marker for the diagnosis and management of prostate cancer. Our BLEIA for PSA, based on the two-step sandwich method, had ultra-high sensitivity and a very wide measurable range. The detection limit (mean of nine replicates of the zero standard +2 SD) for PSA was 0.25 pg/mL and the measurable range for PSA was 0.25 pg/mL–100 ng/mL. Generally, PSA in the serum exists on two forms, called free PSA (f-PSA) and complex PSA (c-PSA), which is formed with α-antichymotripsin. Thus, the response of the PSA assay to these two forms has to be equimolar in the construction of the assay system. Our BLEIA for PSA also had an equimolar response to them. Copyright © 2001 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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