Primary culture of rat ependymal cells in serum-free defined medium
| Autor: | Gérard Labourdette, Marc Weibel, Monique Sensenbrenner, Jean-Claude Artault, Brigitte Pettmann |
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| Rok vydání: | 1986 |
| Předmět: |
Cell type
Ependymal Cell Cell Survival Immunocytochemistry Cell Count Cell Separation Developmental Neuroscience Culture Techniques Ependyma Glial Fibrillary Acidic Protein Animals Bovine serum albumin Molecular Biology Cells Cultured Neurons Glial fibrillary acidic protein biology General Neuroscience Thrombin Rats Inbred Strains Blood Physiological Phenomena Molecular biology In vitro Culture Media Rats Chemically defined medium Animals Newborn Cell culture Immunology Microscopy Electron Scanning biology.protein Neurology (clinical) Developmental Biology |
| Zdroj: | Brain Research. 390:199-209 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/s0006-8993(86)80228-1 |
| Popis: | We have developed a serum-free chemically defined medium which allows the obtainment of a primary culture highly enriched in ciliated ependymal cells. Serum was never used. Mechanically dissociated neonatal rat brain hemisphere cells were seeded on a fibronectin substratum. Culture medium was minimum Eagle's medium until day 14 in vitro and Waymouth's MD 705/l medium thereafter. Both media were supplemented with insulin (5 micrograms/ml), transferrin (10 micrograms/ml) and fatty acid-free bovine serum albumin (0.5 mg/ml). In these conditions, without addition of growth factors, the culture was enriched in ependymal cells; with the addition of thrombin cell growth was stimulated and moreover a nearly pure culture of ependymal cells was obtained. In this very simple culture medium, cells looked healthy up to two months after seeding and about 75% of the cells were ciliated. In a fraction of these cells the glial fibrillary acidic protein, a protein specific for astroglial cells in the central nervous system, was immunohistochemically revealed. The availability of an almost pure primary culture of ependymal cells will make many studies possible on this cell type, particularly studies on the development of these cells, on the regulation of their genetic expression and on their physiological function. |
| Databáze: | OpenAIRE |
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