Polymeric luminol on pre-treated screen-printed electrodes for the design of performant reagentless (bio)sensors

Autor: Loïc J. Blum, Béatrice D. Leca-Bouvier, Audrey Sassolas
Přispěvatelé: Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École Supérieure Chimie Physique Électronique de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Depierre, Frédérique
Rok vydání: 2009
Předmět:
Working electrode
Materials science
Analytical chemistry
02 engineering and technology
01 natural sciences
Luminol
chemistry.chemical_compound
Monolayer
Materials Chemistry
Electrochemiluminescence
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biochemistry [q-bio.BM]
Electrical and Electronic Engineering
[SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biochemistry [q-bio.BM]
Instrumentation
Silica gel
010401 analytical chemistry
Metals and Alloys
Electropolymerization
Choline oxidase
021001 nanoscience & nanotechnology
Condensed Matter Physics
0104 chemical sciences
Surfaces
Coatings and Films
Electronic
Optical and Magnetic Materials
chemistry
Chemical engineering
Screen-printed electrode
Silica sol–gel
Luminophore
0210 nano-technology
Polyluminol
Biosensor
Zdroj: Sensors and Actuators B: Chemical
Sensors and Actuators B: Chemical, Elsevier, 2009, 139, pp.214
ISSN: 0925-4005
DOI: 10.1016/j.snb.2009.01.020
Popis: International audience; A performant reagentless electrochemiluminescent (ECL) system for H2O2 detection based on electropolymerized luminol is first presented. In this work, polyluminol is formed under near-neutral conditions onto pre-treated screen-printed electrodes (SPEs). Pre-treatment conditions of the working electrode surface have been optimized so as to obtain the best ECL responses to H2O2 that were increased by a factor as high as 400. Galvanostatic polymerization has been tested as a new process to form polyluminol films. Good performances were obtained in terms of responses to H2O2, with quite the same linear range as the ones obtained under potentiostatic and potentiodynamic modes.The association of the polyluminol film with an H2O2-producing oxidase has also been investigated, using choline oxidase as a model enzyme. Silica glasses obtained by the sol–gel process have been employed for biomolecule immobilization. Polymeric luminol has been coupled with choline oxidase-immobilizing gel under bilayer or monolayer configurations. In the first case, enzyme has been immobilized in a silica gel formed on a polyluminol film. In the second case, the enzymatic gel formed on SPE was soaked in a solution containing monomeric luminol units, allowing them to diffuse and penetrate throughout the porous gel. Then, electrodeposition was performed to polymerize the luminophore within the silica matrix. In both cases, choline could be detected down to micromolar concentrations.
Databáze: OpenAIRE
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