Development of an Improved ELISA for Antibody Detection and Use in Production of a Hybridoma Secreting a Monoclonal Antibody Specific for Crystal Protein of Bacillus thuringiensis ssp. israelensis
| Autor: | John H. Pruett, Kevin B. Temeyer, Maurice Haufler |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
medicine.drug_class
Bacterial Toxins Bacillus thuringiensis Enzyme-Linked Immunosorbent Assay chemical and pharmacologic phenomena Spleen Monoclonal antibody medicine.disease_cause Cell Line Microbiology Hemolysin Proteins Mice Bacterial Proteins medicine Animals Mice Inbred BALB C Hybridomas Bacillaceae Bacillus thuringiensis Toxins Ecology biology Toxin Antibodies Monoclonal General Medicine biology.organism_classification Bacillales Molecular biology Endotoxins medicine.anatomical_structure Insect Science Monoclonal biology.protein Antibody |
| Zdroj: | Journal of Economic Entomology. 79:1287-1293 |
| ISSN: | 1938-291X 0022-0493 |
| Popis: | Murine hybridomas were constructed by fusing spleen cells from BALB/c mice immunized with soluble crystal protein from Bacillus thuringiensis ssp. israelensis (B.t.i) to the murine myeloma cell line SP2/O-AG14. A direct ELISA (enzyme-linked immunosorbent assay) for detection of antibodies specific for crystal protein of B.t.i. was modified to produce 100- to 1,000-fold increased sensitivity and was used to identify hybridomas secreting monoclonal antibody specific for the B.t.i. crystal protein. Analysis of the hybridoma culture supernatant fluid indicated production of monoclonal IgG3 antibodities, specific for the 68,000 dalton protein presumed to be the insecticidal δ-endotoxin of B.t.i. |
| Databáze: | OpenAIRE |
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