High bioreactor production and emulsifying activity of an unusual exopolymer by Chromohalobacter canadensis 28
| Autor: | Hristo Kisov, Margarita Kambourova, Songul Yasar Yildiz, Nadja Radchenkova, Spasen Vassilev, Ebru Toksoy Oner, Ivanka Boyadzhieva, Nikolina Atanasova, Merve Erginer Hasköylü, Ivan Panchev, Margarita Kuncheva |
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| Přispěvatelé: | Radchenkova, Nadja, Boyadzhieva, Ivanka, Haskoylu, Merve Erginer, Atanasova, Nikolina, Yildiz, Songul Yasar, Kuncheva, Margarita J., Panchev, Ivan, Kisov, Hristo, Vassilev, Spasen, Oner, Ebru Toksoy, Kambourova, Margarita S. |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
exopolymer
Environmental Engineering Exopolymer Liquid paraffin Bioengineering chemistry.chemical_compound halophilic bacterium biocompatibility Bioreactor emulsifying properties Food science Cellulose OPTIMIZATION Research Articles agitation and aeration Guar gum AERATION EXOPOLYSACCHARIDE PRODUCTION AGITATION SPEED chemistry Composition (visual arts) Fermentation Aeration EPS Research Article Biotechnology |
| Zdroj: | Engineering in Life Sciences |
| Popis: | Unusual composition of an exopolymer (EP) from an obligate halophilic bacterium Chromohalobacter canadensis 28 has triggered an interest in development of an effective bioreactor process for its production. Its synthesis was investigated in 2‐L bioreactor at agitation speeds at interval 600‐1000 rpm, at a constant air flow rate of 0.5 vvm; aeration rates of 0.5, 1.0, and 1.5 vvm were tested at constant agitation rate of 900 rpm. EP production was affected by both, agitation and aeration. As a result twofold increase of EP yield was observed and additionally increased up to 3.08 mg/mL in a presence of surfactants. For effective scale‐up of bioreactors mass transfer parameters were estimated and lowest values of KLa obtained for the highest productivity fermentation was established. Emulsification activity of EP exceeded that of trade hydrocolloids xanthan, guar gum, and cellulose. A good synergism between EP and commercial cellulose proved its potential exploration as an enhancer of emulsifying properties of trade emulsions. A pronounced lipophilic effect of EP was established toward olive oil and liquid paraffin. Cultivation of human keratinocyte cells (HaCaT) with crude EP and purified γ‐polyglutamic acid (PGA) showed higher viability than control group. |
| Databáze: | OpenAIRE |
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