Tough aged meat presents greater expression of calpastatin, which presents postmortem protein profile and tenderization related to Nellore steer temperament
Autor: | Giancarlo de Moura Souza, Patricia Maloso Ramos, Steven M. Lonergan, Eduardo Francisquine Delgado, Marcelo Aranda da Silva Coutinho, Giuliana Micai de Oliveira |
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Rok vydání: | 2019 |
Předmět: |
Male
METABOLISMO DE PROTEÍNA High index media_common.quotation_subject Calcium-Binding Proteins 0402 animal and dairy science Protein profile 04 agricultural and veterinary sciences Biology 040401 food science 040201 dairy & animal science Red Meat 0404 agricultural biotechnology Animal science Proteolysis Animals Cattle Temperament Muscle Skeletal Shear Strength Longissimus Lumborum Food Science Calpastatin media_common |
Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
ISSN: | 0309-1740 |
DOI: | 10.1016/j.meatsci.2019.05.017 |
Popis: | Eighteen Nellore steers were classified as either excitable (high index) or calm (low index) temperament, and high or low WB Shear Force (SF) in Longissimus lumborum at 16 days postmortem, with a subset assigned to four groups: HTHS-high temperament and SF (n = 5); HTLS-high temperament and low SF (n = 4); LTHS-low temperament and high SF (n = 4); LTLS-low temperament and SF (n = 5). Only the second calpastatin peak was detected at 48 h in the all samples, and the calpastatin activity only differed (P .05) between shear force groups. High WBSF groups had greater expression of CAST gene (P .05) in the high and low temperament. Western blots showed a calpastatin fragmentation pattern very particular to each group with high molecular weight bands present in the HTHS. Temperament was not a determinant factor in postmortem proteolysis variation. However, greater WBSF aged meat from high temperament animals had greater calpastatin activity and less calpastatin fragments formed during the postmortem period analyzed. |
Databáze: | OpenAIRE |
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