Haemonchus contortus: Evaluation of two signal sequence trapping systems for detection of secreted molecules
| Autor: | Yaping Chen, Debbie Donald, Jody L. Zawadzki, Dadna Hartman |
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| Rok vydání: | 2008 |
| Předmět: |
Signal peptide
Genetic Vectors Immunology Saccharomyces cerevisiae CD13 Antigens Protein Sorting Signals Biology Transfection Chlorocebus aethiops parasitic diseases Animals Secretion Cloning Molecular Integral membrane protein Gene cDNA library Membrane Proteins Helminth Proteins General Medicine biology.organism_classification Cathepsins Molecular biology Yeast Cell biology Infectious Diseases Secretory protein Amylases COS Cells Haemonchus Parasitology Haemonchus contortus |
| Zdroj: | Experimental Parasitology. 119:49-57 |
| ISSN: | 0014-4894 |
| DOI: | 10.1016/j.exppara.2007.12.017 |
| Popis: | Given that signal sequences between secreted proteins of different species can be interchanged, it is reasonable to expect that both mammalian and yeast signal sequence trapping (SST) systems would secrete Haemonchus contortus proteins with similar efficiency and quality. To determine if H. contortus cDNAs that contain a signal sequence could re-establish secretion of a reporter protein, mammalian and yeast SST vectors were designed, 10 H. contortus genes selected, and their respective cDNAs cloned into these two SST vectors. The selected molecules included genes known to code for excretory/secretory or membrane-bound proteins as potential test 'positives', and genes known to code for non-secreted proteins as test 'negatives'. While differentiation between secretion and non-secretion was evident in both systems, the results indicated greater efficiency was achieved when the mammalian system was used. Therefore, mammalian SST using COS cells would be a more useful tool to screen H. contortus cDNA libraries for potential secreted and type-1 integral membrane proteins than yeast SST. |
| Databáze: | OpenAIRE |
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