An automated workflow for the anatomo-functional mapping of the barrel cortex
| Autor: | Guillaume Hucher, Lorraine Perronnet, Daniel E. Shulz, María Eugenia Vilarchao, Isabelle Ferezou, Gabriel Peyré |
|---|---|
| Přispěvatelé: | Unité de Neurosciences Information et Complexité [Gif sur Yvette] (UNIC), Centre National de la Recherche Scientifique (CNRS), CEntre de REcherches en MAthématiques de la DEcision (CEREMADE), Université Paris Dauphine-PSL, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Institut des Neurosciences Paris-Saclay (NeuroPSI), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Méthodes numériques pour le problème de Monge-Kantorovich et Applications en sciences sociales (MOKAPLAN), Inria Paris-Rocquencourt, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), ANR-09-BLAN-0294,TRANSTACT,Transduction mécanique et représentation tactile des textures par les vibrisses de rat et le doigt humain.(2009), ANR-11-JSV4-0004,Sensory Processing,Modulation cholinergique du traitement cortical de l'information sensorielle tactile : couplage de l'imagerie fonctionnelle a l'optogénétique.(2011), European Project: 279593,EC:FP7:ERC,ERC-2011-StG_20101014,SIGMA-VISION(2011), European Project: 269921,EC:FP7:ICT,FP7-ICT-2009-6,BRAINSCALES(2011), Centre National de la Recherche Scientifique (CNRS)-Université Paris Dauphine-PSL, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Peyré, Gabriel, Blanc - Transduction mécanique et représentation tactile des textures par les vibrisses de rat et le doigt humain. - - TRANSTACT2009 - ANR-09-BLAN-0294 - Blanc - VALID, Jeunes Chercheuses et Jeunes Chercheurs - Modulation cholinergique du traitement cortical de l'information sensorielle tactile : couplage de l'imagerie fonctionnelle a l'optogénétique. - - Sensory Processing2011 - ANR-11-JSV4-0004 - JCJC - VALID, Sparsity, Image and Geometry to Model Adaptively Visual Processings - SIGMA-VISION - - EC:FP7:ERC2011-10-01 - 2016-09-30 - 279593 - VALID, Brain-inspired multiscale computation in neuromorphic hybrid systems - BRAINSCALES - - EC:FP7:ICT2011-01-01 - 2015-03-31 - 269921 - VALID |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Computer science [INFO.INFO-TS] Computer Science [cs]/Signal and Image Processing Interface (computing) [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology Barrel (horology) histological sections Sensory system Workflow blood vessels robust iterative closest point 03 medical and health sciences Mice 0302 clinical medicine [INFO.INFO-TS]Computer Science [cs]/Signal and Image Processing registration Physical Stimulation medicine Animals Computer vision [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] Rigid transformation Brain Mapping business.industry voltage sensitive dye imaging General Neuroscience [SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology Iterative closest point Numerical Analysis Computer-Assisted Somatosensory Cortex Barrel cortex Voltage-Sensitive Dye Imaging 030104 developmental biology medicine.anatomical_structure Cytoarchitecture Cerebral cortex Vibrissae barrel cortex [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] Artificial intelligence business 030217 neurology & neurosurgery gradient domain fusion |
| Zdroj: | Journal of Neuroscience Methods Journal of Neuroscience Methods, 2016, pp.11. ⟨10.1016/j.jneumeth.2015.09.008⟩ Journal of Neuroscience Methods, Elsevier, 2016, pp.11. ⟨10.1016/j.jneumeth.2015.09.008⟩ |
| ISSN: | 0165-0270 |
| DOI: | 10.1016/j.jneumeth.2015.09.008⟩ |
| Popis: | International audience; Highlights: – Here is a new tool to map functional data onto the barrel cortex structure. – It realigns histological slices and reconstructs the barrel map in 2-D. – Slice realignment by rigid transformations is computed using detected blood vessels. – Barrel map reconstruction is obtained by gradient fusion. – Its application is exemplified for voltage sensitive dye imaging experiments. Background: The rodent barrel cortex is a widely used model to study the cortical processing of tactile sensory information. It is notable by the cytoarchitecture of its layer IV, which contains distinguishable structural units called barrels that can be considered as anatomical landmarks of the functional columnar organization of the cerebral cortex. To study sensory integration in the barrel cortex it is therefore essential to map recorded functional data onto the underlying barrel topography, which can be reconstructed from the post hoc alignment of tangential brain slices stained for cytochrome oxidase. New Method: This article presents an automated workflow to perform the registration of histological slices of the barrel cortex followed by the 2-D reconstruction of the barrel map from the registered slices. The registration of two successive slices is obtained by computing a rigid transformation to align sets of detected blood vessel cross-sections. This is achieved by using a robust variant of the classical iterative closest point method. A single fused image of the barrel field is then generated by computing a nonlinear merging of the gradients from the registered images. Comparison with Existing Methods: This novel anatomo-functional mapping tool leads to a substantial gain in time and precision compared to conventional manual methods. It provides a flexible interface for the user with only a few parameters to tune. Conclusions: We demonstrate here the usefulness of the method for voltage sensitive dye imaging of the mouse barrel cortex. The method could also benefit other experimental approaches and model species. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect