Nerve growth factor protects R2 cells against neurotoxicity induced by methamphetamine
| Autor: | Jian-Hui Liang, Ji-Wang Zheng, Chang-ling Li, Jun-lan Zhou |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
medicine.medical_specialty
Programmed cell death Cell Survival Apoptosis Pharmacology Toxicology Cell Line Methamphetamine Mice Internal medicine Cerebellum Nerve Growth Factor medicine Animals Viability assay biology Neurotoxicity General Medicine medicine.disease Flow Cytometry Rats Nerve growth factor Endocrinology Neuroprotective Agents biology.protein DNA fragmentation Neurotrophin medicine.drug |
| Zdroj: | Toxicology letters. 150(2) |
| ISSN: | 0378-4274 |
| Popis: | It is well known that methamphetamine is a neurotoxic drug of abuse to cause cell death both in vitro and in vivo. In this study, the protective effect of nerve growth factor on methamphetamine-induced neurotoxicity was examined in R2 cells. Treatment of R2 cells with methamphetamine for 48 h induced apoptotic-like death as determined by cell viability assay, DNA fragmentation on agarose gel and flow cytometric analysis. Co-treatment of R2 cells with nerve growth factor (10-1000 U/ml) in the presence of methamphetamine increased cell viability, decreased the intensity of methamphetamine-induced DNA fragmentation and reduced the number of apoptotic cells with flow cytometry. These results indicate that nerve growth factor may be useful for the treatment of methamphetamine users. |
| Databáze: | OpenAIRE |
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