Generation and characterization of novel co-stimulatory anti-mouse TNFR2 antibodies
| Autor: | Segués, Aina, van Duijnhoven, Sander M J, Parade, Marc, Driessen, Lilian, Vukovic, Nataša, Zaiss, Dietmar, Sijts, Alice J A M, Berraondo, Pedro, van Elsas, Andrea, LS Immunologie, Immunologie, dI&I RA-I&I I&I |
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| Přispěvatelé: | LS Immunologie, Immunologie, dI&I RA-I&I I&I |
| Rok vydání: | 2021 |
| Předmět: |
Immunology
CHO Cells medicine.disease_cause Epitope Antibodies Autoimmunity Rats Sprague-Dawley 03 medical and health sciences Mice 0302 clinical medicine Immune system Cricetulus cysteine-rich domain antibody medicine Immunology and Allergy Animals Receptors Tumor Necrosis Factor Type II Antibody 030304 developmental biology Autoimmune disease 0303 health sciences epitope treg biology Chemistry medicine.disease 3. Good health Cell biology Rats Treg Cysteine-rich domain TNFR2 Costimulation costimulation 030220 oncology & carcinogenesis biology.protein Tumor necrosis factor alpha Female Tumor necrosis factor receptor 2 Ex vivo |
| Zdroj: | Journal of Immunological Methods Segués, A, Van Duijnhoven, S M J, Parade, M, Driessen, L, Vukovic, N, Zaiss, D, Sijts, A J A M, Berraondo, P & Van Elsas, A 2021, ' Generation and characterization of novel co-stimulatory anti-mouse TNFR2 antibodies ', Journal of Immunological Methods, vol. 499, 113173 . https://doi.org/10.1016/j.jim.2021.113173 Journal of Immunological Methods, 499, 1. Elsevier |
| ISSN: | 1872-7905 0022-1759 |
| DOI: | 10.1016/j.jim.2021.113173 |
| Popis: | Tumor necrosis factor receptor 2 (TNFR2) has gained much research interest in recent years because of its potential pivotal role in autoimmune disease and cancer. However, its function in regulating different immune cells is not well understood. There is a need for well-characterized reagents to selectively modulate TNFR2 function, thereby enabling definition of TNFR2-dependent biology in human and mouse surrogate models. Here, we describe the generation, production, purification, and characterization of a panel of novel antibodies targeting mouse TNFR2. The antibodies display functional differences in binding affinity and potency to block TNFα. Furthermore, epitope binding showed that the anti-mTNFR2 antibodies target different domains on the TNFR2 protein, associated with varying capacity to enhance CD8+ T-cell activation and costimulation. Moreover, the anti-TNFR2 antibodies demonstrate binding to isolated splenic mouse Tregs ex vivo and activated CD8+ cells, reinforcing their potential use to establish TNFR2-dependent immune modulation in translational models of autoimmunity and cancer. |
| Databáze: | OpenAIRE |
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