Synaptojanin 1 is required for endolysosomal trafficking of synaptic proteins in cone photoreceptor inner segments

Autor: Eva Y. Ma, Lars C. Holzhausen, Sachihiro C. Suzuki, Susan E. Brockerhoff, Ashley A. George, Sara Hayden
Rok vydání: 2013
Předmět:
Anatomy and Physiology
genetic structures
Synaptobrevin
Visual System
Golgi Apparatus
Endoplasmic Reticulum
Animals
Genetically Modified
Molecular Cell Biology
Neurobiology of Disease and Regeneration
health care economics and organizations
Zebrafish
Multidisciplinary
Neuronal Morphology
Animal Models
Cellular Structures
Sensory Systems
Cell biology
Protein Transport
symbols
Retinal Cone Photoreceptor Cells
Medicine
Membranes and Sorting
Synaptic Vesicles
Research Article
Retinal Photoreceptor Cell Outer Segment
Endosome
Science
Nerve Tissue Proteins
Endosomes
Biology
Synaptic vesicle
symbols.namesake
Model Organisms
Ocular System
Autophagy
Animals
Retinal Photoreceptor Cell Inner Segment
Cilia
Endoplasmic reticulum
Golgi apparatus
Phosphoric Monoester Hydrolases
Subcellular Organelles
Cellular Neuroscience
sense organs
Lysosomes
Neuroscience
Zdroj: PLoS ONE
PLoS ONE, Vol 9, Iss 1, p e84394 (2014)
ISSN: 1932-6203
Popis: Highly polarized cells such as photoreceptors require precise and efficient strategies for establishing and maintaining the proper subcellular distribution of proteins. The signals and molecular machinery that regulate trafficking and sorting of synaptic proteins within cone inner segments is mostly unknown. In this study, we show that the polyphosphoinositide phosphatase Synaptojanin 1 (SynJ1) is critical for this process. We used transgenic markers for trafficking pathways, electron microscopy, and immunocytochemistry to characterize trafficking defects in cones of the zebrafish mutant, nrc(a14) , which is deficient in phosphoinositide phosphatase, SynJ1. The outer segments and connecting cilia of nrc(a14) cone photoreceptors are normal, but RibeyeB and VAMP2/synaptobrevin, which normally localize to the synapse, accumulate in the nrc(a14) inner segment. The structure of the Endoplasmic Reticulum in nrc(a14) mutant cones is normal. Golgi develop normally, but later become disordered. Large vesicular structures accumulate within nrc(a14) cone photoreceptor inner segments, particularly after prolonged incubation in darkness. Cone inner segments of nrc (a14) mutants also have enlarged acidic vesicles, abnormal late endosomes, and a disruption in autophagy. This last pathway also appears exacerbated by darkness. Taken altogether, these findings show that SynJ1 is required in cones for normal endolysosomal trafficking of synaptic proteins.
Databáze: OpenAIRE
Externí odkaz: