Polymorphism C242T in the Cyp19 gene in meat sheep
Autor: | Fernanda Tanamati, N. H. A. P. Mora, Eliane Gasparino, S.C.C. Silva, Francisco de Assis Fonseca de Macedo, G. P. Schuroff |
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Jazyk: | angličtina |
Předmět: |
0301 basic medicine
Veterinary medicine Genotype Biology Bioinformatics Cyp19 polymorphism 03 medical and health sciences Aromatase Gene Frequency Santa Inês Polymorphism (computer science) lcsh:Botany lcsh:Zoology Animals lcsh:QL1-991 lcsh:Science lcsh:QH301-705.5 Polymorphism Genetic Sheep White Dorper polimorfismo Dorper Breed Genotype frequency lcsh:QK1-989 genomic DNA Restriction enzyme 030104 developmental biology lcsh:Biology (General) Herd Texel lcsh:Q General Agricultural and Biological Sciences |
Zdroj: | Brazilian Journal of Biology, Vol 76, Iss 1, Pp 205-208 Brazilian Journal of Biology v.76 n.1 2016 Brazilian Journal of Biology Instituto Internacional de Ecologia (IIE) instacron:IIE Brazilian Journal of Biology, Volume: 76, Issue: 1, Pages: 205-208, Published: 22 JAN 2016 |
ISSN: | 1678-4375 |
Popis: | The objective of this study was to evaluate the frequency of C242T polymorphism on the aromatase gene and the allelic and genotypic frequency of these variants in sheep belonging to four breed groups. Blood samples were collected from 187 animals of four breed groups: Dorper, Santa Inês, Texel and White Dorper, originated from herds in the region of Maringá/PR, Brazil. The genomic DNA was extracted using alkaline extraction, with subsequent amplification of the fragments via PCR with specific primer. The samples resulting from amplification were subjected to digestion process using the Dpn II restriction enzyme and to polyacrylamide gel electrophoresis 10.0% and stained with silver nitrate. Three distinct genotypes were observed: homozygous (CC), heterozygous (CT) and homozygous for no cut (TT). The resulting data were analyzed using the POPGENE software with 5% significance. Genotypic frequencies among the breed groups were: Texel (CC - 0.426; CT - 0.511; TT - 0.064), Dorper (CC - 0.073; CT - 0.732; TT - 0.439), White Dorper (CC - 0.021; CT - 0.255; TT - 0.723) and Santa Inês (CC - 0.115; CT - 0.462; TT - 0.423). Resumo O objetivo deste trabalho foi avaliar as frequências alélicas e genotípicas do polimorfismo do C242T no gene da aromatase em ovinos pertencentes a quatro grupos raciais. Foram coletadas amostras de sangue de 187 animais de quatro grupos raciais: Dorper, Santa Inês, Texel e White Dorper, provenientes de rebanhos da região de Maringá, PR - BR. O DNA genômico foi extraído utilizando o método de extração alcalina, com posterior amplificação dos fragmentos via PCR com primer específico. As amostras resultantes da amplificação foram submetidas ao processo de digestão com auxilio da enzima restrição Dpn II e submetido à eletroforese em gel de poliacrilamida de 10,0% e corado nitrato de prata. Foram observados três genótipos distintos: Homozigoto (CC), heterozigoto (CT) e homozigoto para não corte (TT). Os dados resultantes foram analisados utilizando o software POPGENE com significância de 5%. As frequências genotípicas entre os grupos raciais foram: Texel (CC - 0,426; CT - 0,511; TT - 0,064), Dorper (CC - 0,073; CT - 0,732; TT - 0,439), White Dorper (CC - 0,021; CT - 0,255; TT - 0,723) e Santa Inês (CC - 0,115; CT - 0,462; TT - 0,423). |
Databáze: | OpenAIRE |
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