Glassy dynamics, cell mechanics, and endothelial permeability
| Autor: | Ramaswamy Krishnan, Kavitha Rajendran, Jeffrey J. Fredberg, C. Corey Hardin, Christopher V. Carman, Greeshma Manomohan, Roberta Martinelli, Dhananjay T. Tambe, James P. Butler |
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| Rok vydání: | 2013 |
| Předmět: |
Cell Membrane Permeability
Pyridines Cell Phase Transition Article Cell Line Thrombin Sphingosine Materials Chemistry medicine Humans Physical and Theoretical Chemistry Cytoskeleton Fluorescent Dyes Microscopy Confocal Chemistry Hepatocyte Growth Factor Endothelial Cells Hydrogen Peroxide Amides Surfaces Coatings and Films Endothelial stem cell medicine.anatomical_structure Biochemistry Rho kinase inhibitor Permeability (electromagnetism) Biophysics Hepatocyte growth factor Lysophospholipids Intracellular medicine.drug Histamine |
| Zdroj: | The journal of physical chemistry. B. 117(42) |
| ISSN: | 1520-5207 |
| Popis: | A key feature of all inflammatory processes is disruption of the vascular endothelial barrier. Such disruption is initiated in part through active contraction of the cytoskeleton of the endothelial cell (EC). Because contractile forces are propagated from cell to cell across a great many cell-cell junctions, this contractile process is strongly cooperative and highly nonlocal. We show here that the characteristic length scale of propagation is modulated by agonists and antagonists that impact permeability of the endothelial barrier. In the presence of agonists including thrombin, histamine, and H2O2, force correlation length increases, whereas in the presence of antagonists including sphingosine-1-phosphate, hepatocyte growth factor, and the rho kinase inhibitor, Y27632, force correlation length decreases. Intercellular force chains and force clusters are also evident, both of which are reminiscent of soft glassy materials approaching a glass transition. |
| Databáze: | OpenAIRE |
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