DNA hybridization in nanostructural molecular assemblies enables detection of gene mutations without a fluorescent probe
| Autor: | Toshimitsu Shinohara, Tatsuo Maruyama, Masahiro Goto, Lian Chun Park |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Polymers and Plastics
DNA Mutational Analysis Bioengineering Gene mutation Biology medicine.disease_cause Polymorphism Single Nucleotide Biomaterials Materials Chemistry medicine Humans Nanotechnology Point Mutation A-DNA Micelles Mutation Oligonucleotide Hybridization probe DNA–DNA hybridization Point mutation Nucleic Acid Hybridization Molecular biology Fluorescence Biophysics Solvents Spectrophotometry Ultraviolet Tumor Suppressor Protein p53 Oligonucleotide Probes |
| Zdroj: | Biomacromolecules. 5(1) |
| ISSN: | 1525-7797 |
| Popis: | We have developed a simple single nucleotide polymorphisms (SNPs) analysis utilizing DNA hybridization in nanostructural molecular assemblies. The novel technique enables the detection of a single-base mismatch in a DNA sequence without a fluorescent probe. This report describes for the first time that DNA hybridization occurs in the nanostructural molecular assemblies (termed reverse micelles) formed in an organic medium. The restricted nanospace in the reverse micelles amplifies the differences in the hybridization rate between mismatched and perfectly matched DNA probes. For a model system, we hybridized a 20-mer based on the p53 gene sequence to 20-mer complementary oligonucleotides with various types of mismatches. Without any DNA labeling or electrochemical apparatus, we successfully detected the various oligonucleotide mismatches by simply measuring the UV absorbance at 260 nm. |
| Databáze: | OpenAIRE |
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