| Autor: |
Breno H, Almeida, Mário L S, Medeiros, Ana C D S, Bezerra, Michele D C, Silva |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
International Journal of Biological Macromolecules. 200:409-415 |
| ISSN: |
0141-8130 |
| DOI: |
10.1016/j.ijbiomac.2022.01.077 |
| Popis: |
Haemonchus contortus is a hematophagous parasite that causes damage to ruminant production worldwide. This study reported the in vitro nematicidal effect of a lectin preparation (LP) isolated from Artocarpus heterophyllus seeds on larvae and adults of H. contortus. The protein extraction was in phosphate-buffered saline followed by protein precipitation with ammonium sulfate 70% and dialysis. The dialyzed protein fraction was chromatographed to obtain isolated LP. The LP was characterized by hemagglutinating activity (HA) assays, protein dosage and polyacrylamide gel electrophoresis. The motility index of H. contortus in the third larval stage (unsheathed L3 larvae) and adult stage was evaluated. The HA inhibition of LP by mannose and galactose as well as the electrophoretic profile indicated the presence of the lectins ArtinM and Jacalin. The motility index of H. contortus was significantly reduced (p 0.001) during the first 8 h of LP exposure, both in larvae (lowest index 8.3% with LP at 1 mg mL |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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