Cellular immune response of lymph nodes from dogs following the intradermal injection of a recombinant antigen corresponding to a 66 kDa protein of Echinococcus granulosus
| Autor: | T. Marchal, Y Fu, I. Saint-André Marchal, A.F Petavy, G Bosquet |
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| Rok vydání: | 2000 |
| Předmět: |
Injections
Intradermal CD3 Immunology Antibodies Helminth law.invention Immune system Dogs law medicine Animals Echinococcus granulosus Lymph node Immunity Cellular General Veterinary biology Germinal center Helminth Proteins biology.organism_classification Flow Cytometry Immunohistochemistry Lymphocyte Subsets Recombinant Proteins Echinococcus medicine.anatomical_structure Antigens Helminth biology.protein Recombinant DNA Lymph Lymph Nodes CD8 |
| Zdroj: | Veterinary immunology and immunopathology. 74(3-4) |
| ISSN: | 0165-2427 |
| Popis: | A recombinant polypeptide (referred to as EgA31), which represents a 66 kDa protein, was prepared from an Echinococcus granulosus cDNA library. In order to assess its potential to induce cellular immune responses, dog popliteal and prescapular lymph nodes were sensitized with this recombinant polypeptide. Subpopulations of lymphocytes were then analyzed by flow cytometry and immunohistochemistry on lymph node sections. Five days after the sensitization, the paracortical areas of the lymph nodes appeared hypertrophic, the number of CD3+, CD4+, CD8+ and CD5+ cells increased, the number of B-cells began to augment and some secondary follicles occurred, and a number of CD4+ cells appeared in germinal centers. Many large secondary follicles and a significantly augmented number of CD5+ cells in cords of medullae were observed 10 days after the sensitization. These active cellular responses strengthen the interest for further studies on the development of a vaccine with this recombinant polypeptide. |
| Databáze: | OpenAIRE |
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