A bovine model of a respiratoryParachlamydia acanthamoebaeinfection
| Autor: | Gilbert Greub, Carola Ostermann, Markus Lohr, Elisabeth M. Liebler-Tenorio, Wieland Schroedl, Petra Reinhold, Annette Prohl, Sébastien Aeby |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Microbiology (medical) Polymerase Chain Reaction Severity of Illness Index Parenchyma Parachlamydia acanthamoebae Pneumonia Bacterial medicine Animals Immunology and Allergy Prospective Studies Respiratory system Lung Chlamydiales General Immunology and Microbiology biology Respiratory disease Acute-phase protein General Medicine biology.organism_classification medicine.disease Immunohistochemistry Bacterial Load Disease Models Animal Pneumonia Infectious Diseases medicine.anatomical_structure Parachlamydia Immunology Cattle Gram-Negative Bacterial Infections |
| Zdroj: | Pathogens and Disease, vol. 73, no. 1, pp. 1-14 |
| ISSN: | 2049-632X |
| DOI: | 10.1111/2049-632x.12201 |
| Popis: | The aim of this study was to evaluate the pathogenicity of Parachlamydia (P.) acanthamoebae as a potential agent of lower respiratory tract disease in a bovine model of induced lung infection. Intrabronchial inoculation with P. acanthamoebae was performed in healthy calves aged 2-3 months using two challenge doses: 10(8) and 10(10) bacteria per animal. Controls received 10(8) heat-inactivated bacteria. Challenge with 10(8) viable Parachlamydia resulted in a mild degree of general indisposition, whereas 10(10) bacteria induced a more severe respiratory illness becoming apparent 1-2 days post inoculation (dpi), affecting 9/9 (100%) animals and lasting for 6 days. The extent of macroscopic pulmonary lesions was as high as 6.6 (6.0)% [median (range)] of lung tissue at 2-4 dpi and correlated with parachlamydial genomic copy numbers detected by PCR, and with bacterial load estimated by immunohistochemistry in lung tissue. Clinical outcome, acute phase reactants, pathological findings and bacterial load exhibited an initial dose-dependent effect on severity. Animals fully recovered from clinical signs of respiratory disease within 5 days. The bovine lung was shown to be moderately susceptible to P. acanthamoebae, exhibiting a transient pneumonic inflammation after intrabronchial challenge. Further studies are warranted to determine the precise pathophysiologic pathways of host-pathogen interaction. |
| Databáze: | OpenAIRE |
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