Effects of Levonorgestrel and progesterone on Oviductal physiology in mammals
| Autor: | Yan Liang, Ya-Jing Tan, Duo Zhang, Gui-Lin Liang, He-Feng Huang, Huiyu Zhang, Qian Zhu, Jian Zhang, Hang Qi, Wei Xia, Xiaoqing He, Zhen Huang, Rui-Hong Xue, Jiang-Jing Yuan, Cheng Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male endocrine system animal structures lcsh:QH471-489 Receptivity Physiology Oviducts Levonorgestrel lcsh:Gynecology and obstetrics 03 medical and health sciences Mice 0302 clinical medicine Endocrinology Organ Culture Techniques In vivo Cell Line Tumor medicine Contraceptive Agents Female Animals lcsh:Reproduction Progesterone lcsh:RG1-991 Pregnancy 030219 obstetrics & reproductive medicine Ectopic pregnancy Dose-Response Relationship Drug business.industry urogenital system Research Ciliary beat frequency Obstetrics and Gynecology Embryo Oviduct medicine.disease female genital diseases and pregnancy complications Transplantation Mice Inbred C57BL 030104 developmental biology Reproductive Medicine Female business Developmental Biology medicine.drug |
| Zdroj: | Reproductive Biology and Endocrinology, Vol 16, Iss 1, Pp 1-8 (2018) Reproductive Biology and Endocrinology : RB&E |
| ISSN: | 1477-7827 |
| Popis: | Background Our previous study indicated that emergency contraception, including levonorgestrel and progesterone, could lead to ectopic pregnancy following contraception failure. However, our understanding of the effects of levonorgestrel and progesterone on oviductal physiology is limited. Methods The receptivity of the fallopian tubal epithelium after levonorgestrel and progesterone treatment was examined through western blots for receptivity markers and JAr-spheroid-fallopian tubal epithelial cell attachment assays. The ciliary beat frequency was analyzed using an inverted bright-field microscope. Furthermore, an in vivo animal model of embryo-tubal transplantation was also studied to determine the effects of levonorgestrel- and progesterone-induced ciliary beat reduction. Results Our results showed that levonorgestrel and progesterone did not change the levels of fallopian tubal epithelial cell receptive markers, including LIF, STAT3, IGFBP1, ITGB3, MUC1, and ACVR1B, or affect JAr-spheroid implantation. However, levonorgestrel and progesterone reduced the ciliary beat frequency in fallopian tubes in a dose-dependent manner. An in vivo model also showed that levonorgestrel and progesterone could lead to embryo retention in the oviducts. Conclusions These findings show that levonorgestrel and progesterone can reduce the ciliary beat frequency without altering receptivity, indicating a possible mechanism for progesterone- or levonorgestrel-induced tubal pregnancy. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |