Immunophenotyping of Non-Hodgkin's Lymphoma in Paraffin-Embedded Tissue Sections: A Comparison with Genotypic Analysis
| Autor: | Duggan Db, Betty A. Forbes, Frederick R. Davey, M T Elghetany, Schuehler K, Anthony S. Kurec |
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| Rok vydání: | 1991 |
| Předmět: |
Gene Rearrangement
B-Lymphocytes Pathology medicine.medical_specialty Lineage (genetic) Genotype Lymphoma Non-Hodgkin Antibodies Monoclonal General Medicine Gene rearrangement Biology medicine.disease Immunophenotyping Lymphoma Non-Hodgkin's lymphoma Blotting Southern Antigen medicine Humans Immunoglobulin heavy chain Gene Rearrangement beta-Chain T-Cell Antigen Receptor DNA Probes Immunoglobulin Heavy Chains |
| Zdroj: | American Journal of Clinical Pathology. 95:517-525 |
| ISSN: | 1943-7722 0002-9173 |
| DOI: | 10.1093/ajcp/95.4.517 |
| Popis: | Several monoclonal antibodies (MoAbs) are now available for immunophenotyping non-Hodgkin's lymphomas (NHLs) in paraffin-embedded tissue sections. To determine the reliability of these reagents in predicting the genotype, 44 cases of NHL were studied with the alkaline phosphatase-anti-alkaline phosphatase technique with the use of the following MoAbs: leukocyte common antigen (CD45), Mac 387, L26, 4KB5, MB1, MB2, LN2, UCHL1, MT1, and MT2. The lineage of the neoplastic cells was determined in all cases by gene rearrangement studies for immunoglobulin heavy chain and for the T-cell receptor beta-chain. Genotypic results showed B-cell lineage in 33 cases (75%), T-cell lineage in 6 cases (14%), and mixed or undetermined lineage in 5 cases (11%). A concordance of lineage assignment by paraffin section immunophenotyping with gene rearrangement studies was observed in 37 of 39 (95%) lymphomas with an unequivocally defined genotype. MoAb L26 was the most sensitive in detecting B-cell genotype; MoAbs MT1 and UCHL1 were the most sensitive and specific, respectively, in detecting T-cell genotype. The authors conclude that lineage assignment of NHLs in paraffin sections is reflective of the corresponding genotype when an appropriate panel of MoAbs is used. |
| Databáze: | OpenAIRE |
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