Docosahexaenoic and Eicosapentaenoic Acids Segregate Differently between Raft and Nonraft Domains
| Autor: | Benjamin Drew Rockett, Stephen R. Wassall, Shawn E. Batten, Justin A. Williams, Mitchel Harris, William Stillwell, Saame Raza Shaikh |
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| Rok vydání: | 2012 |
| Předmět: |
Magnetic Resonance Spectroscopy
Docosahexaenoic Acids Detergents Biophysics Oleic Acids Biology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Membrane Microdomains Lipid raft 030304 developmental biology chemistry.chemical_classification 0303 health sciences Cholesterol Temperature Membrane food and beverages Membranes Artificial Raft Fish oil Eicosapentaenoic acid Sphingomyelins chemistry Biochemistry Eicosapentaenoic Acid Docosahexaenoic acid Phosphatidylcholines lipids (amino acids peptides and proteins) Sphingomyelin 030217 neurology & neurosurgery Polyunsaturated fatty acid |
| Zdroj: | Biophysical Journal. 103(2):228-237 |
| ISSN: | 0006-3495 |
| DOI: | 10.1016/j.bpj.2012.06.016 |
| Popis: | Omega-3 polyunsaturated fatty acids (n-3 PUFA), enriched in fish oils, are increasingly recognized to have potential benefits for treating many human afflictions. Despite the importance of PUFA, their molecular mechanism of action remains unclear. One emerging hypothesis is that phospholipids containing n-3 PUFA acyl chains modify the structure and composition of membrane rafts, thus affecting cell signaling. In this study the two major n-3 PUFA found in fish oils, eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, are compared. Using solid-state (2)H NMR spectroscopy we explored the molecular organization of 1-[(2)H(31)]palmitoyl-2-eicosapentaenoylphosphatidylcholine (PEPC-d(31)) and 1-[(2)H(31)]palmitoyl-2-docosahexaenoylphosphatidylcholine (PDPC-d(31)) in mixtures with sphingomyelin (SM) and cholesterol (chol). Our results indicate that whereas both PEPC-d(31) and PDPC-d(31) can accumulate into SM-rich/chol-rich raftlike domains, the tendency for DHA to incorporate into rafts is more than twice as great as for EPA. We propose that DHA may be the more bioactive component of fish oil that serves to disrupt lipid raft domain organization. This mechanism represents an evolution in the view of how PUFA remodel membrane architecture. |
| Databáze: | OpenAIRE |
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