Synthetic periprosthetic synovial fluid development for in vitro cell‐tribocorrosion testing using the Taguchi array approach
| Autor: | Jeremy Mercuri, Piyush Khullar, Jeremy L. Gilbert, Michael J Wiegand |
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| Rok vydání: | 2020 |
| Předmět: |
Materials science
Cell Survival Tribocorrosion 0206 medical engineering Cell Culture Techniques Biomedical Engineering 02 engineering and technology Biomaterials Mice Taguchi methods chemistry.chemical_compound In vivo Synovial Fluid Hyaluronic acid Animals Synovial fluid Hyaluronic Acid Hydrogen peroxide Phospholipids Chromatography Macrophages Metals and Alloys Albumin 021001 nanoscience & nanotechnology Glutathione 020601 biomedical engineering In vitro Corrosion RAW 264.7 Cells chemistry Ceramics and Composites 0210 nano-technology |
| Zdroj: | Journal of Biomedical Materials Research Part A. 109:551-561 |
| ISSN: | 1552-4965 1549-3296 |
| DOI: | 10.1002/jbm.a.37039 |
| Popis: | Synovial fluid is dynamic in vivo with biological components changing in ratio and size depending on the health of the joint space, making it difficult to model in vitro. Previous efforts to develop synthetic synovial fluid have typically focused on single organic-tribological interactions with implant surfaces, thus ignoring interplay between multiple solution components. Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response. While no single synovial fluid variable significantly affected the collective response, HA:PL ratio resulted in the largest impact factor (Δ) on 12 of the 13 measured responses with significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface. Cluster analysis separated significant responses from all trials into three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions. By utilizing the Taguchi approach in combination with cluster analysis, we were able to advance our knowledge of complex multivariate synthetic synovial fluids influence on macrophage and electrochemical behavior at the cell-solution-metal interface. |
| Databáze: | OpenAIRE |
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