Meiotic behavior and H3K4m distribution in B chromosomes of Characidium gomesi (Characiformes, Crenuchidae)
| Autor: | Claudio Oliveira, Elkin Y. Suárez-Villota, Érica Alves Serrano, Cristian Araya-Jaime, Fausto Foresti |
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| Přispěvatelé: | Universidade Estadual Paulista (UNESP), Universidad Austral de Chile, Instituto Butantan |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine CharaciformesAnimalia Heterochromatin Plant Science Biology 01 natural sciences Bivalent (genetics) 03 medical and health sciences Chromosome painting Meiosis Genetics Animalia Synaptonemal complex Chordata Small supernumerary marker chromosome Mitosis Research Articles Immunodetection B chromosome Crenuchidae Actinopterygii Characidium gomesiAnimalia synaptonemal complex biology.organism_classification 030104 developmental biology Characidium Animal Science and Zoology Characiformes SYCP3 010606 plant biology & botany Biotechnology |
| Zdroj: | Comparative Cytogenetics Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Comparative Cytogenetics 10(2): 255-268 |
| ISSN: | 1993-078X 1993-0771 |
| Popis: | Made available in DSpace on 2022-04-29T08:27:14Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-01-01 Characidium gomesi Travasso, 1956 specimens from the Pardo River have up to four heterochromatic supernumerary chromosomes, derived from the sex chromosomes. To access the meiotic behavior and distribution of an active chromatin marker, males and females of C. gomesi with two or three B chromosomes were analyzed. Mitotic chromosomes were characterized using C-banding and FISH with B chromosome probes. Meiocytes were subjected to immunofluorescence-FISH assay using anti-SYCP3, anti-H3K4m, and B chromosomes probes. Molecular homology of supernumeraries was confirmed by FISH and by its bivalent conformation in individuals with two of these chromosomes. In individuals with three Bs, these elements formed a bivalent and a univalent. Supernumerary and sex chromosomes exhibited H3K4m signals during pachytene contrasting with their heterochromatic and asynaptic nature, which suggest a more structural role than functional of this histone modification. The implications of this result are discussed in light of the homology, meiotic nuclear organization, and meiotic silencing of unsynapsed chomatin. Departamento de Morfologia Instituto de Biociências Universidade Estadual Paulista, Distrito de Rubião Junior, s/n Instituto de Ciencias Marinas y Limnólogicas Universidad Austral de Chile, Casilla 567 Laboratório de Ecologia e Evolução Instituto Butantan, Avenida Vital Brazil, 1500, São Paulo Departamento de Morfologia Instituto de Biociências Universidade Estadual Paulista, Distrito de Rubião Junior, s/n |
| Databáze: | OpenAIRE |
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