Impact of gonadotropins on oocyte maturation, fertilisation and developmental competence in vitro
| Autor: | Zhan Zhang, Tony Tsai, Xuemei Wang, Jie Qiao, Huai L Feng |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
medicine.drug_class
Fertilization in Vitro Reproductive technology In Vitro Techniques Biology Oogenesis Human chorionic gonadotropin Andrology Endocrinology Genetics medicine Animals Molecular Biology Cumulus Cells Dose-Response Relationship Drug Embryo culture Anatomy Oocyte medicine.anatomical_structure Reproductive Medicine Oocytes Cattle Animal Science and Zoology Folliculogenesis Gonadotropin Gonadotropins hormones hormone substitutes and hormone antagonists Fetal bovine serum Developmental Biology Biotechnology |
| Zdroj: | Reproduction, Fertility and Development. 26:752 |
| ISSN: | 1031-3613 |
| Popis: | The aim of the present study was to evaluate the dose-dependent effects of gonadotropins, either singly (Bravelle (B), Luveris (L), Menupur (M), Repronex (R), Gonal-F (G), Follism (F) and Norvarel (N)) or in combination (Menupur + Bravelle; Repronext + Bravelle; and Bravelle + Norvarel), on rates of oocyte maturation, fertilisation and early embryo development in vitro in an animal model. Bovine cumulus–oocyte complexes (COCs) were purchased commercially and cultured in TCM-199 with 10% fetal bovine serum supplemented with varying concentrations of gonadotropin (0, 5, 10, 20, 40 IU or United States Pharmacopoeia (USP) mL–1) for 24 and 48 h according to current IVF clinical stimulation protocols. All gonadotropins enhanced oocyte maturation in vitro in a dose-dependent manner. Individually, Gonal-F (Merck KGaA, Darmstadt, Germany), Follism (Merck Co, Whitehouse Station, NJ, USA) and Repronext (Ferring, Parsippany, NJ, USA) promoted oocyte maturation; in combination, they effectively enhanced COC expansion and increased the maturation competence of MII oocytes. However, high concentrations of gonadotropins may result in maturation arrest. Specific combinations of gonadotropins may change the rate of early embryonic development (8–16-cells) and morula–blastocyst formation. These data provide support for the responsiveness of bovine oocytes to gonadotropins in vitro and the need to consider variations in the relative concentrations and ratio of combinations (FSH/LH or human chorionic gonadotropin) for optimisation of oocyte developmental competence. The results of the present study could be applied to therapeutic clinical stimulation protocols and help improve IVF success rates. |
| Databáze: | OpenAIRE |
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