Transglutaminase 2 limits murine peritoneal acute gout-like inflammation by regulating macrophage clearance of apoptotic neutrophils
| Autor: | Anya D. Sydlaske, Kristen Johnson, Robert Terkeltaub, Amir Agha-Babakhani, David M. Rose |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Gout Tissue transglutaminase Neutrophils Phagocytosis Neutrophile Immunology Peritonitis Inflammation Apoptosis Biology Mice Rheumatology GTP-Binding Proteins Transforming Growth Factor beta Extracellular medicine Immunology and Allergy Macrophage Animals Pharmacology (medical) Protein Glutamine gamma Glutamyltransferase 2 Cells Cultured Mice Knockout Transglutaminases medicine.disease Molecular biology Uric Acid Gene Expression Regulation biology.protein Macrophages Peritoneal Female medicine.symptom |
| Zdroj: | Arthritis and rheumatism. 54(10) |
| ISSN: | 0004-3591 |
| Popis: | Objective Monosodium urate monohydrate (MSU) crystals have remarkable inflammatory potential. However, gouty inflammation is spontaneously self-limited, an occurrence recognized since antiquity. Gouty synovitis is driven and sustained by neutrophil influx. Importantly, macrophage phagocytosis of apoptotic (but not necrotic) neutrophils is antiinflammatory. Therefore, we tested the hypothesis that efficient clearance of apoptotic neutrophils by macrophages is one of the factors that restrains the progression of gouty inflammation. Macrophage expression of transglutaminase 2 (TG2), a multifunctional protein with reciprocally regulated transamidation and purine nucleotide–binding activities, promotes apoptotic leukocyte uptake. In this study, we tested the specific role of macrophage TG2 expression in MSU crystal–induced inflammation. Methods We studied MSU crystal–induced peritonitis in TG2−/− and congenic TG2+/+ mice. We also studied the effects of TG2 on apoptotic cell uptake by cultured macrophages. Results TG2−/− mice demonstrated more progressive neutrophilic accumulation than did TG2+/+ mice, which was associated with delayed clearance of apoptotic neutrophils during MSU crystal–induced peritonitis. We observed defective phagocytosis of apoptotic leukocytes by TG2−/− peritoneal macrophages, which was corrected by soluble extracellular TG2. Transamidation catalytic activity of TG2 was not required to mediate macrophage uptake of apoptotic leukocytes. In contrast, the TG2 nucleotide binding site residue K173 was critical for this TG2 function. TG2 bound to GDP, ADP, or ATP (but not to GTP) rescued defective apoptotic leukocyte uptake by TG2−/− macrophages. Conclusion Enhancement of apoptotic neutrophil uptake by macrophage-derived TG2 restrains gout-like neutrophilic peritoneal inflammation. Differential binding of TG2 by purine nucleotides may contribute to clinical variability in the extent and duration of gouty inflammation. |
| Databáze: | OpenAIRE |
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