Comparison of Milk Fat Globule Membrane (MFGM) Proteins of Chianina and Holstein Cattle Breed Milk Samples Through Proteomics Methods
| Autor: | Silvia Bongiorni, Lorraine Pariset, Anna Maria Timperio, Alessio Valentini, Lello Zolla, Leonardo Murgiano |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Proteomics
lcsh:TX341-641 lactation Biology Article Glycolipid fluids and secretions Lactation Lipid droplet milk proteomics MFGM cattle MS/MS medicine bestiame Animals Secretion Food science Glycoproteins chemistry.chemical_classification Nutrition and Dietetics Gene Expression Profiling food and beverages Membrane Proteins Lipid Droplets Breed medicine.anatomical_structure Biochemistry chemistry Membrane protein Gene Expression Regulation Female proteomica del latte lattazione Glycolipids Glycoprotein lcsh:Nutrition. Foods and food supply Food Science |
| Zdroj: | Nutrients Nutrients, Vol 1, Iss 2, Pp 302-315 (2009) Nutrients; Volume 1; Issue 2; Pages: 302-315 |
| ISSN: | 2072-6643 |
| Popis: | Identification of proteins involved in milk production is important to understand the biology of lactation. Many studies have advanced the understanding of mammary function and milk secretion, but the critical molecular mechanisms implicated in milk fat secretion is still incomplete. Milk Fat Globules are secreted from the apical surface of the mammary cells, surrounded by a thin membrane bilayer, the Milk Fat Globule Membrane (MFGM), formed by proteins which have been suggested to be cholesterolemia-lowering factors, inhibitors of cancer cell growth, vitamin binders, bactericidal, suppressors of multiple sclerosis. Using a proteomic approach, we compared MFGM from milk samples of individuals belonging to two different cattle breeds, Chianina and Holstein, representative of selection for milk and meat traits, respectively. We were able to isolate some of the major MFGM proteins in the examined samples and to identify differences between the protein fractions of the two breeds. We detected differences in the amount of proteins linked to mammary gland development and lipid droplets formation, as well as host defence mechanisms. We have shown that proteomics is a suitable, unbiased method for the study of milk fractions proteins and a powerful tool in nutritional genomics. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|