Freezing human peripheral lymphocytes and a technique for culture in monolayers
| Autor: | Charles R. Plank, Lon R. White, Martha M. Harlem, Jacob A. Brody |
|---|---|
| Rok vydání: | 1968 |
| Předmět: |
food.ingredient
Chemistry Petri dish Tritium Tuberculin Molecular biology Gelatin General Biochemistry Genetics and Molecular Biology Peripheral law.invention food Antigen Lymphocyte transformation law Culture Techniques Lectins Monolayer Immunology Freezing Methods Vaccinia Lymphocytes Previously treated |
| Zdroj: | Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.). 129(3) |
| ISSN: | 0037-9727 |
| Popis: | SummaryA successful, relatively inexpensive method is described for freezing peripheral lymphocytes. These lymphocytes when thawed and grown in the presence of antigens transformed at approximately the same rate as unfrozen control cultures from the same individuals. A method is also described for successfully culturing lymphocytes which adhere to the surfaces of cover slips or petri plates previously treated with 0.5% gelatin. Rates of lymphocyte transformation in these cultures were similar to those encountered in culture tubes. |
| Databáze: | OpenAIRE |
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