Rapid separation and identification of 31 major saponins in Shizhu ginseng by ultra-high performance liquid chromatography-electron spray ionization-MS/MS
| Autor: | Xingjie Guo, Xinlei Liang, He-Yun Zhu, Ting-Ting Sun, Xu-Ling Peng, Longshan Zhao |
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| Rok vydání: | 2015 |
| Předmět: |
separation
Chromatography Traditional medicine 010405 organic chemistry Chemistry 010401 analytical chemistry 01 natural sciences Biochemistry Genetics and Molecular Biology (miscellaneous) lcsh:QK1-989 0104 chemical sciences Shizhu ginseng Ginseng Complementary and alternative medicine ultra-high performance liquid chromatography coupled with MS/MS lcsh:Botany Ionization saponins identification Ultra high performance Biotechnology Research Article |
| Zdroj: | Journal of Ginseng Research Journal of Ginseng Research, Vol 40, Iss 3, Pp 220-228 (2016) |
| ISSN: | 1226-8453 |
| Popis: | Background: Among the various ginseng strains, Shizhu ginseng is endemic to China, mainly distributed in Kuandian Manchu Autonomous County (Liaoning Province, China); however, not much is known about the compounds (especially saponins) in Shizhu ginseng. Methods: A rapid, sensitive, and reliable ultra-high performance liquid chromatography coupled with MS/MS (UHPLC–MS/MS) method was developed to separate and identify saponins in Shizhu ginseng. Results: The separation was carried out on a Waters ACQUITY UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm) with acetonitrile and 0.1% formic acid aqueous solution as the mobile phase under a gradient elution at 40°C. The detection was performed on a Micromass Quattro Micro API mass spectrometer equipped with electrospray ionization source in both positive and negative modes. Under the optimized conditions, a total of 31 saponins were identified or tentatively characterized by comparing retention time and MS data with related literatures and reference substances. Conclusion: The developed UHPLC–MS/MS method was suitable for identifying and characterizing the chemical constituents in Shizhu ginseng, which provided a helpful chemical basis for further research on Shizhu ginseng. |
| Databáze: | OpenAIRE |
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