Application of a Vital Fluorescent Staining Method for Simultaneous, Near-Real-Time Concentration Monitoring of Two Bacterial Strains in an Atlantic Coastal Plain Aquifer in Oyster, Virginia
| Autor: | Pengfei Zhang, Brian J. Mailloux, Mark E. Fuller, J. Hall, William P. Kovacik, Tullis C. Onstott, Mary F. DeFlaun, Sheryl H. Streger, William P. Johnson, Simon Vainberg |
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| Rok vydání: | 2004 |
| Předmět: |
Geologic Sediments
Oyster Coastal plain Colony Count Microbial Succinimides Aquifer Applied Microbiology and Biotechnology Comamonadaceae chemistry.chemical_compound TRACER biology.animal Methods Enumeration Seawater Fluorescein Ecosystem Fluorescent Dyes Comamonas geography geography.geographical_feature_category Chromatography Staining and Labeling Ecology biology Rhodamines Chemistry Virginia Fluoresceins biology.organism_classification Biodegradation Environmental Environmental Pollutants Water Microbiology Environmental Monitoring Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 70:1680-1687 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.70.3.1680-1687.2004 |
| Popis: | Two differentially labeled bacterial strains were monitored in near-real time during two field-scale bacterial transport experiments in a shallow aquifer in July 2000 and July 2001. Comamonas sp. strain DA001 and Acidovorax sp. strain OY-107 were grown and labeled with the vital fluorescent stain TAMRA/SE (5 [and -6]-carboxytetramethylrhodamine, succinimidyl ester) or CFDA/SE (5 [and -6]-carboxyfluorescein diacetate, succinimidyl ester). Fluorescently labeled cells and a conservative bromide tracer were introduced into a suboxic superficial aquifer, followed by groundwater collection from down-gradient multilevel samplers. Cells were enumerated in the field by microplate spectrofluorometry, with confirmatory analyses for selected samples done in the laboratory by epifluorescence microscopy, flow cytometry, and ferrographic capture. There was general agreement in the results from all of the vital-stain-based enumeration methods, with differences ranging from |
| Databáze: | OpenAIRE |
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