Detection of E. coli labeled with metal-conjugated antibodies using lateral-flow assay and laser-induced breakdown spectroscopy

Autor:	Xianglei Mao, Prasoon K. Diwakar, Jennifer Sturgis, Larry H. Stanker, Valery Patsekin, Bartek Rajwa, Iyll-Joon Doh, Vassilia Zorba, Euiwon Bae, Eva Biela, Cole Reynolds, Carmen Gondhalekar, J. Paul Robinson, Richard E. Russo
Rok vydání:	2020
Předmět:	Materials science chemistry.chemical_element 02 engineering and technology Conjugated system 01 natural sciences Biochemistry Analytical Chemistry Metal Escherichia coli detection Escherichia coli medicine Laser-induced breakdown spectroscopy chemistry.chemical_classification Detection limit Chromatography medicine.diagnostic_test Lasers Spectrum Analysis Biomolecule 010401 analytical chemistry 021001 nanoscience & nanotechnology Antibodies Bacterial 0104 chemical sciences chemistry Metals Immunoassay visual_art visual_art.visual_art_medium 0210 nano-technology Europium
Zdroj:	Analytical and Bioanalytical Chemistry. 412:1291-1301
ISSN:	1618-2650 1618-2642
DOI:	10.1007/s00216-019-02347-3
Popis:	This study explores the adoption of laser-induced breakdown spectroscopy (LIBS) for the analysis of lateral-flow immunoassays (LFIAs). Gold (Au) nanoparticles are standard biomolecular labels among LFIAs, typically detected via colorimetric means. A wide diversity of lanthanide-complexed polymers (LCPs) are also used as immunoassay labels but are inapt for LFIAs due to lab-bound detection instrumentation. This is the first study to show the capability of LIBS to transition LCPs into the realm of LFIAs, and one of the few to apply LIBS to biomolecular label detection in complete immunoassays. Initially, an in-house LIBS system was optimized to detect an Au standard through a process of line selection across acquisition delay times, followed by determining limit of detection (LOD). The optimized LIBS system was applied to Au-labeled Escherichia coli detection on a commercial LFIA; comparison with colorimetric detection yielded similar LODs (1.03E4 and 8.890E3 CFU/mL respectively). Optimization was repeated with lanthanide standards to determine if they were viable alternatives to Au labels. It was found that europium (Eu) and ytterbium (Yb) may be more favorable biomolecular labels than Au. To test whether Eu-complexed polymers conjugated to antibodies could be used as labels in LFIAs, the conjugates were successfully applied to E. coli detection in a modified commercial LFIA. The results suggest interesting opportunities for creating highly multiplexed LFIAs. Multiplexed, sensitive, portable, and rapid LIBS detection of biomolecules concentrated and labeled on LFIAs is highly relevant for applications like food safety, where in-field food contaminant detection is critical. Graphical abstract.
Databáze:	OpenAIRE
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