The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome

Autor: Linda Dowd, Theresa Feltwell, Tracey Chillingworth, Stephen Baker, Zahra Hance, Brendan W. Wren, Richard A. Stabler, Nicholas R. Thomson, S. Holroyd, Anne Wright, Audrey Fraser, Sarah Sharp, Adam P. Roberts, Karen Brooks, Michael A. Quail, K. Stevens, Kay Jagels, Peter Mullany, Louise Unwin, Paul Davis, Hongmei Wang, Claire Price, Bart Barrell, Carol Churcher, Ann Cronin, Matthew T. G. Holden, Ana Cerdeño-Tárraga, Nathalie Bason, Neil F. Fairweather, Karen Mungall, Mark Simmonds, Nigel P. Minton, Julian Parkhill, Ester Rabbinowitsch, Sally Whithead, Gordon Dougan, Bruno Dupuy, Mohammed Sebaihia, Sharon Moule
Přispěvatelé: The Wellcome Trust Sanger Institute [Cambridge], Department of Infectious and Tropical Diseases (LSHTM), London School of Hygiene and Tropical Medicine (LSHTM), Eastman Dental Institute [UCL, London], University College of London [London] (UCL), MRC Centre for Molecular Microbiology and Infection [Imperial College, London] (CMBI), Imperial College London, Centre for Biomolecular Sciences [Nottingham, UK] (CBS), University of Nottingham, UK (UON), Génétique Moléculaire Bactérienne, Institut Pasteur [Paris] (IP), This work was supported by the Wellcome Trust., We would like to acknowledge the support of the Wellcome Trust Sanger Institute core sequencing and informatics groups. We thank D. Gerding and G. Songer for provision of C. difficile strains, F. Barbut and J. Emerson for help with the antibiotic susceptibility tests, and the BUGs microarray facility at St. George's Hospital for provision of the C. difficile 630 microarray., Institut Pasteur [Paris]
Rok vydání: 2006
Předmět:
[SDV]Life Sciences [q-bio]
MESH: Base Sequence
MESH: Genome
Bacterial
Genome
Drug Resistance
Multiple
Bacterial
MESH: Clostridium difficile/pathogenicity
MESH: Clostridium difficile/physiology
MESH: Spores
Bacterial/physiology
Enterocolitis
Pseudomembranous
MESH: Gastrointestinal Tract/microbiology
Oligonucleotide Array Sequence Analysis
Spores
Bacterial
Genetics
0303 health sciences
Virulence
Mosaicism
Clostridium difficile
MESH: Enterocolitis
Pseudomembranous/etiology
Adaptation
Physiological
Conjugation
Genetic
MESH: Mosaicism
DNA
Bacterial
Transposable element
Molecular Sequence Data
Biology
MESH: Bacterial Proteins/genetics
MESH: Clostridium difficile/drug effects
MESH: Enterocolitis
Pseudomembranous/microbiology
MESH: DNA Transposable Elements/genetics
03 medical and health sciences
Bacterial Proteins
Humans
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
MESH: Virulence/genetics
MESH: Drug Resistance
Multiple
Bacterial/genetics
Gene
030304 developmental biology
Whole genome sequencing
MESH: Humans
MESH: Molecular Sequence Data
Base Sequence
Clostridioides difficile
030306 microbiology
Microarray analysis techniques
MESH: Conjugation
Genetic
MESH: Adaptation
Physiological
MESH: DNA
Bacterial/genetics
Gastrointestinal Tract
MESH: Oligonucleotide Array Sequence Analysis
DNA Transposable Elements
Mobile genetic elements
MESH: Clostridium difficile/genetics
Genome
Bacterial
Zdroj: Nature Genetics
Nature Genetics, 2006, 38 (7), pp.779-786. ⟨10.1038/ng1830⟩
Nature Genetics, Nature Publishing Group, 2006, 38 (7), pp.779-786. ⟨10.1038/ng1830⟩
ISSN: 1546-1718
1061-4036
DOI: 10.1038/ng1830
Popis: International audience; We determined the complete genome sequence of Clostridium difficile strain 630, a virulent and multidrug-resistant strain. Our analysis indicates that a large proportion (11%) of the genome consists of mobile genetic elements, mainly in the form of conjugative transposons. These mobile elements are putatively responsible for the acquisition by C. difficile of an extensive array of genes involved in antimicrobial resistance, virulence, host interaction and the production of surface structures. The metabolic capabilities encoded in the genome show multiple adaptations for survival and growth within the gut environment. The extreme genome variability was confirmed by whole-genome microarray analysis; it may reflect the organism's niche in the gut and should provide information on the evolution of virulence in this organism.
Databáze: OpenAIRE
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