N-Stearoylethanolamine suppresses the pro-inflammatory cytokines production by inhibition of NF-κB translocation
| Autor: | Rostislav R. Panchuk, Hula Nm, Rostislav S. Stoika, Andrey G. Berdyshev, O V Onopchenko, Kosiakova Hv |
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| Rok vydání: | 2015 |
| Předmět: |
Lipopolysaccharides
endocrine system Physiology Active Transport Cell Nucleus Anti-Inflammatory Agents Chromosomal translocation Inflammation Biology Biochemistry Proinflammatory cytokine chemistry.chemical_compound Mice Insulin resistance Cell Line Tumor medicine Animals Insulin Obesity RNA Messenger Pharmacology Cell Nucleus Dose-Response Relationship Drug Body Weight NF-kappa B NF-κB Cell Biology medicine.disease Molecular biology Rats Leukemia nervous system chemistry Ethanolamines Immunology Macrophages Peritoneal L1210 cells Cytokines Tumor necrosis factor alpha medicine.symptom Insulin Resistance Stearic Acids |
| Zdroj: | Prostaglandinsother lipid mediators. 121 |
| ISSN: | 1098-8823 |
| Popis: | N-Stearoylethanolamine (NSE) is a minor lipid that belongs to the N-Acylethanolamines family that mediates a wide range of biological processes. This study investigates the mechanisms of anti-inflammatory action of NSE on different model systems. Namely, we estimated the effect of NSE on inflammatory cytokines mRNA level (leukemia cells L1210), cytokines content (serum and LPS-stimulated macrophages) and nuclear translocation of NF-κB (peritoneal macrophages LPS-stimulated and isolated from rats with obesity-induced insulin resistance). The results indicated that NSE dose-dependently inhibits the IL-1 and IL-6 mRNA level in L1210 cells. Furthermore, the NSE treatment triggered a normalization of serum TNF-α level in insulin resistant rats and a reduction of medium IL-1 level in LPS-activated peritoneal macrophages. These NSE's effects were associated with the inhibition of nuclear NF-κB translocation in rat peritoneal macrophages. |
| Databáze: | OpenAIRE |
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