Progesterone reverts LPS-reduced FAAH activity in murine peripheral blood mononuclear cells by a receptor-mediated fashion
| Autor: | Julieta Aisemberg, Manuel Luis Wolfson, Claudia Alejandra Vercelli, Ana Paula Domínguez Rubio, Ana Inés Salazar, Ana Maria Franchi |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Lipopolysaccharides
Male medicine.medical_specialty CIENCIAS MÉDICAS Y DE LA SALUD Lipopolysaccharide T-Lymphocytes 2-Arachidonoylglycerol Gene Expression Ciencias de la Salud Biology Pregnant Biochemistry Peripheral blood mononuclear cell Amidohydrolases Mice chemistry.chemical_compound Endocrinology Pregnancy Fatty acid amide hydrolase Internal medicine Progesterone receptor medicine Animals Molecular Biology Lonaprisan Progesterone Mice Inbred BALB C Anandamide Monoacylglycerol lipase Otras Ciencias de la Salud nervous system chemistry Peripheral blood mononuclear cells Immunology Leukocytes Mononuclear Female lipids (amino acids peptides and proteins) Receptors Progesterone psychological phenomena and processes |
| Popis: | Increased anandamide concentrations are associated with pregnancy failure. Anandamide levels are regulated by the fatty acid amide hydrolase (FAAH). The aim of the study was to investigate the role of progesterone (P) on FAAH modulation in murine peripheral blood mononuclear cells (PBMC) under septic conditions. We observed that in vivo administration of LPS to non-pregnant (NP) mice decreased FAAH activity of PBMC while in pregnant mice no changes in FAAH activity were observed. NP animals administered with P had a similar response to LPS as the pregnant animals. Also, NP mice injected with P antagonist and P showed that the effect of P on LPS-reduced FAAH activity was impaired. Furthermore, LPS produced a decrease in the ratio of PR-B/PR-A in NP animals. Our results showed that, in our model the endotoxin decreased PBMC’s FAAH activity and this condition was reverted by P in a receptor-mediated fashion. Fil: Wolfson, Manuel Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina Fil: Aisemberg, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina Fil: Salazar, Ana Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina Fil: Domínguez Rubio, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina Fil: Vercelli, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires; Argentina Fil: Franchi, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina |
| Databáze: | OpenAIRE |
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