Nifedipine inhibits activation of transcription factor NF-κB
| Autor: | Youichi Nunokawa, Shigetake Sasayama, Akira Matsumori |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Lipopolysaccharides
Lung Neoplasms Nifedipine Pharmacology Lymphocyte Activation Transfection General Biochemistry Genetics and Molecular Biology Diltiazem chemistry.chemical_compound Tumor Cells Cultured medicine Humans Electrophoretic mobility shift assay General Pharmacology Toxicology and Pharmaceutics Luciferases A549 cell Dose-Response Relationship Drug Tumor Necrosis Factor-alpha Macrophages Calcium channel NF-kappa B Epithelial Cells Promoter General Medicine Calcium Channel Blockers Verapamil chemistry Cell Migration Inhibition Phorbol Tetradecanoylphorbol Acetate Amlodipine Interleukin-1 medicine.drug |
| Zdroj: | Life Sciences. 67:2655-2661 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/s0024-3205(00)00849-3 |
| Popis: | This study was performed to examine the effects of the calcium channel blockers, nifedipine, amlodipine, diltiazem, and verapamil on the activation of the transcription factor NF-kappaB. A549 cells, a human epithelium-like lung carcinoma cell line, were transfected with the NF-kappaB reporter plasmid, which contains the luciferase gene driven by promoters containing a TATA element and 5 copies of the kappaB cis-acting element, and co-transfected with 0.2 microg of pSV2neo vector using LipofectAMINE. Nifedipine significantly decreased the expression of luciferase protein stimulated with IL-1beta (1 ng/mL) compared with controls: 80+/-4% at 3 micromol/L, 47+/-2% at 10 micromol/L and 30+/-2% at 30 micromol/L (each, n=3, p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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