Endocytosis and Digestion in Carnivorous Pitcher Plants of the Family Sarraceniaceae
Autor: | Stefanie Krammer, Anselm Pavlik, Manfred Edlinger, Wolfram Adlassnig, Ingeborg Lang, Marianne Koller-Peroutka |
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Rok vydání: | 2019 |
Předmět: |
0106 biological sciences
0301 basic medicine Proteases Darlingtonia medicine.medical_treatment Heliamphora nutans Plant Science carnivorous plants digestion Endocytosis 010603 evolutionary biology 01 natural sciences Article Heliamphora 03 medical and health sciences medicine endocytosis pitcher traps Ecology Evolution Behavior and Systematics Sarracenia Protease Darlingtonia californica Ecology biology Chemistry fungi methylene-blue Botany biology.organism_classification 030104 developmental biology Biochemistry QK1-989 uptake Sarraceniaceae FITC-BSA |
Zdroj: | Plants Volume 8 Issue 10 Plants, Vol 8, Iss 10, p 367 (2019) |
ISSN: | 2223-7747 |
Popis: | Highly evolved carnivorous plants secrete digestive enzymes for degradation of trapped animals and absorb whole macromolecules from their prey by means of endocytosis. (1) Background: In the pitcher-plant family Sarraceniaceae, the production of enzymes is dubious and no evidence for endocytosis is known so far. (2) Methods: Heliamphora nutans, Darlingtonia californica, and nine taxa of Sarracenia are tested for cuticular pores, and for protease and endocytosis of the fluorescent protein analogue FITC-BSA, after 10&ndash 48 h of stimulation. (3) Results: Cuticular pores as a prerequisite for enzyme secretion and nutrient uptake are present in all tested species. Permeable cells form clusters in the inner epidermis of the pitchers, but are only little differentiated from impermeable epidermis cells. Proteases are found in S. psittacina, S. leucophylla, S. minor, S. oreophila, S. alabamensis, H. nutans, D. californica lacking only in S. flava and in S. purpurea ssp. purpurea, S. purpurea ssp. venosa, S. rosea, where enzyme production is possibly replaced by degradation via the extraordinary diverse inquiline fauna. S. leucophylla, S. minor, S. oreophila exhibit both protease production and endocytosis S. psittacina, S. alabamensis, H. nutans, D. californica produce proteases only no single species shows endocytosis without protease production. (4) Conclusions: Protease secretion seems to be a prerequisite for endocytotic nutrient uptake. Transport of FITC-BSA absorbed by endocytosis towards the vascular tissue of the trap leaves suggests that endocytosis of nutrients is more than a side effect of enzyme secretion. |
Databáze: | OpenAIRE |
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