Tools for resistance monitoring in oriental fruit moth (Lepidoptera: Tortricidae) and first assessment in Brazilian populations
| Autor: | Benoît Sauphanor, Myriam Siegwart, Lino Bittencourt Monteiro, Sandrine Maugin, S. Malfitano Carvalho, Jérôme Olivares |
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| Přispěvatelé: | Unité de recherche Plantes et Systèmes de Culture Horticoles (PSH), Institut National de la Recherche Agronomique (INRA), Universidade Federal do Paraná (UFPR), Departamento de Entomologia, Universidade Federal de Lavras (UFLA), Companha de Aperfeicoamento de Pessoal de Nivel Superior (Brazil) |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
0106 biological sciences
Insecticides Pyridines Thiazines Moths Carbaryl 01 natural sciences Genetic analysis Toxicology Insecticide Resistance chemistry.chemical_compound Neonicotinoids Pyrethrins résistance aux pesticides amérique du sud prunus persica malus domestica 0303 health sciences brésil Ecology biology Strain (biology) activité enzymatique General Medicine [SHS.ECO]Humanities and Social Sciences/Economics and Finance Cydia molesta acétylcholinestérase Horticulture Hydrazines Malus Acetylcholinesterase Chlorpyrifos europe marqueur moléculaire france Brazil Tortricidae séquence nucleique pommier Lepidoptera genitalia Economies et finances 03 medical and health sciences Nitriles Animals 030304 developmental biology arbre fruitier malus domestica pommier arbre fruitier prunus persica insecticidebrésil amérique du sud france europerésistance aux pesticides activité enzymatiqueséquence nucleique marqueur moléculaireacétylcholinestérase insecticide Organothiophosphorus Compounds Sequence Analysis DNA biology.organism_classification Resistance monitoring 010602 entomology Economies and finances Deltamethrin chemistry Insect Science |
| Zdroj: | Journal of Economic Entomology Journal of Economic Entomology, Entomological Society of America, 2011, 104 (2), pp.636-645. ⟨10.1603/EC10302⟩ Journal of Economic Entomology 2 (104), 636-645. (2011) |
| ISSN: | 0022-0493 |
| DOI: | 10.1603/EC10302⟩ |
| Popis: | Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699; International audience; In southern Brazilian apple (Malus spp.) orchards, predominantly organophosphates are used to control the oriental fruit moth, Cydia molesta (Busck) (Lepidoptera: Tortricidae), but control failures often occur. Therefore the susceptibility of three C. molesta Brazilian populations was investigated to five insecticides of different groups and modes of action, in comparison with a susceptible laboratory strain mass reared in southern France for >10 yr. At the same time, comparative biochemical and genetic analysis were performed, assessing the activities of the detoxification enzymatic systems and sequencing a gene of insecticide molecular target to find out markers associated with resistance. The three Brazilian populations were significantly resistant to chlorpyrifos ethyl compared with the reference strain. One of the field populations that had been frequently exposed to deltamethrin treatments showed significant decreasing susceptibility to this compound, whereas none of the three populations had loss of susceptibility to tebufenozide and thiacloprid compared with the reference strain. All three populations had slight but significant increases of glutathione transferase and carboxylesterases activities and significant decrease of specific acetylcholinesterase activities compared with the reference. Only the most resistant population to chlorpyriphos exhibited a significantly higher mixed function oxidase activity than the reference. The acetylcholinesterase of females was significantly less inhibited by carbaryl in the Brazilian populations than in the reference strain (1.7-2.5-fold), and this difference was not expressed in the male moth. However, no mutation in the MACE locus was detected. These biological and molecular characterizations of adaptive response to insecticides in C. molesta provide tools for early detection of insecticide resistance in field populations of this pest |
| Databáze: | OpenAIRE |
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