Induction of apoptosis and necrosis in lymphocytes by the cis-Pt(II) complex of 3-aminoflavone in comparison with cis-DDP
| Autor: | Justyn Ochocki, Leszek Szmigiero, Regina Osiecka, Elzbieta Zyner, Ewa Ciesielska, Beata Kosmider |
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| Rok vydání: | 2004 |
| Předmět: |
Flavonoids
A549 cell Necrosis TUNEL assay Health Toxicology and Mutagenesis Apoptosis Biology chemistry.chemical_compound Microscopy Fluorescence chemistry Immunology Toxicity In Situ Nick-End Labeling Genetics Cancer research medicine Humans Female Trypan blue Propidium iodide Cisplatin medicine.symptom Cytotoxicity |
| Zdroj: | Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 558:169-179 |
| ISSN: | 1383-5718 |
| Popis: | cis-Diamminedichloroplatinum(II) (cis-DDP) is one of the most widely administrated antitumor drugs. However, the use of cis-DDP is severely limited because of its toxic side effects. Therefore, efforts are concentrated on the development of improved platinum compounds with a broader activity spectrum and effectiveness in chemotherapy, but lower toxicity. Beneficial properties of flavonoids, e.g. their antitumor activity, encouraged scientists to synthesize cis-bis(3-aminoflavone)dichloroplatinum(II). Abilities of these compounds to induce apoptosis and necrosis were compared by use of trypan blue, fluorochrome staining (Hoechst 33258/propidium iodide double staining) and TUNEL assays. The cytotoxicity was evaluated by MTT. The results obtained show that the cis-Pt(II) complex of 3-aminoflavone is less toxic than cis-DDP. However, the former compound has a faster rate of apoptosis induction in lymphocytes than the latter. The cis-Pt(II) complex of 3-aminoflavone induces apoptosis in normal lymphocytes to a lesser degree and could be a potential antitumor drug. |
| Databáze: | OpenAIRE |
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