18Fluorodeoxyglucose Accumulation in Arterial Tissues Determined by PET Signal Analysis
| Autor: | Michael Winterdahl, Zahra P. Nasr, Zheer Al-Mashhadi, Martin M. Bjørklund, Jacob F. Bentzon, Lars Poulsen Tolbod, Jørgen Frøkiær, Rozh H Al-Mashhadi, Erling Falk, Lars Ø Bloch |
|---|---|
| Přispěvatelé: | Danish Council for Independent Research, Lundbeck Foundation, Danish Heart Foundation, Aarhus University (Dinamarca), Ministerio de Ciencia, Innovación y Universidades (España), Fundación ProCNIC |
| Rok vydání: | 2019 |
| Předmět: |
ATHEROSCLEROTIC PLAQUE INFLAMMATION
Pathology medicine.medical_specialty PET/CT HYPOXIA 030204 cardiovascular system & hematology fluorodeoxyglucose Lesion 03 medical and health sciences 0302 clinical medicine Smooth muscle medicine Distribution (pharmacology) signal model 030212 general & internal medicine Clinical imaging MACROPHAGES FDG-PET Fluorodeoxyglucose PET-CT medicine.diagnostic_test business.industry Fdg uptake INHIBITOR carbohydrates (lipids) WALL INFLAMMATION Positron emission tomography atherosclerosis medicine.symptom Cardiology and Cardiovascular Medicine business medicine.drug |
| Zdroj: | Al-Mashhadi, R H, Tolbod, L P, Bloch, L, Bjørklund, M M, Nasr, Z P, Al-Mashhadi, Z, Winterdahl, M, Frøkiær, J, Falk, E & Bentzon, J F 2019, ' 18 Fluorodeoxyglucose Accumulation in Arterial Tissues Determined by PET Signal Analysis ', Journal of the American College of Cardiology, vol. 74, no. 9, pp. 1220-1232 . https://doi.org/10.1016/j.jacc.2019.06.057 Repisalud Instituto de Salud Carlos III (ISCIII) |
| ISSN: | 0735-1097 |
| DOI: | 10.1016/j.jacc.2019.06.057 |
| Popis: | BACKGROUND: Arterial 18fluorodeoxyglucose (FDG) positron emission tomography (PET) is considered a measure of atherosclerotic plaque macrophages and is used for quantification of disease activity in clinical trials, but the distribution profile of FDG across macrophages and other arterial cells has not been fully clarified. OBJECTIVES: The purpose of this study was to analyze FDG uptake in different arterial tissues and their contribution to PET signal in normal and atherosclerotic arteries. METHODS: Wild-type and D374Y-PCSK9 transgenic Yucatan minipigs were fed a high-fat, high-cholesterol diet to induce atherosclerosis and subjected to a clinical FDG-PET and computed tomography scan protocol. Volumes of arterial media, intima/lesion, macrophage-rich, and hypoxic tissues were measured in serial histological sections. Distributions of FDG in macrophages and other arterial tissues were quantified using modeling of the in vivo PET signal. In separate transgenic minipigs, the intra-arterial localization of FDG was determined directly by autoradiography. RESULTS: Arterial FDG-PET signal appearance and intensity were similar to human imaging. The modeling approach showed high accuracy in describing the FDG-PET signal and revealed comparable FDG accumulation in macrophages and other arterial tissues, including medial smooth muscle cells. These findings were verified directly by autoradiography of normal and atherosclerotic arteries. CONCLUSIONS: FDG is taken up comparably in macrophage-rich and -poor arterial tissues in minipigs. This offers a mechanistic explanation to a growing number of observations in clinical imaging studies that have been difficult to reconcile with macrophage-selective FDG uptake. This study was supported by the Danish Council for Independent Research/Medical Sciences, Lundbeck Foundation, Danish Heart Foundation, and Aarhus University Research Foundation (AU IDEAS). The CNIC is supported by the Ministerio de Ciencia, Innovación y Universidades, and the Pro CNIC Foundation; and is a Severo Ochoa Center of Excellence (SEV-2015-0505). Dr. Bentzon has served as a consultant for Novo Nordisk A/S; and has within the last 5 years received an investigator-initiated preclinical research grant from Regeneron Pharmaceuticals Sí |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|