Single-cell transcriptome analysis of CD8 + T-cell memory inflation
| Autor: | C Willberg, C de Lara, Madeleine Zinser, Chansavath Phetsouphanh, Claire Hutchings, Emanuele Marchi, Andrew J Highton, Lian Ni Lee, Paul Klenerman, Claire L. Gordon |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Medicine (miscellaneous)
Spleen Biology General Biochemistry Genetics and Molecular Biology Viral vector 03 medical and health sciences 0302 clinical medicine memory T cells Gene expression medicine Cytotoxic T cell resident-memory T cells cytomegalovirus Conventional memory memory inflation 030304 developmental biology 0303 health sciences adenovirus vector RNA Articles 3. Good health Gut Epithelium Cell biology medicine.anatomical_structure CD8 030215 immunology Research Article |
| Zdroj: | Wellcome Open Research |
| ISSN: | 2398-502X |
| Popis: | Background: Persistent viruses such as murine cytomegalovirus (MCMV) and adenovirus-based vaccines induce strong, sustained CD8+ T-cell responses, described as memory “inflation”. These retain functionality, home to peripheral organs and are associated with a distinct transcriptional program. Methods: To further define the nature of the transcriptional mechanisms underpinning memory inflation at different sites we used single-cell RNA sequencing of tetramer-sorted cells from MCMV-infected mice, analyzing transcriptional networks in virus-specific populations in the spleen and gut intra-epithelial lymphocytes (IEL). Results: We provide a transcriptional map of T-cell memory and define a module of gene expression, which distinguishes memory inflation in spleen from resident memory T-cells (TRM) in the gut. Conclusions: These data indicate that CD8+ T-cell memory in the gut epithelium induced by persistent viruses and vaccines has a distinct quality from both conventional memory and “inflationary” memory which may be relevant to protection against mucosal infections. |
| Databáze: | OpenAIRE |
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