Fatty Acid Uptake in T Cell Subsets Using a Quantum Dot Fatty Acid Conjugate
| Autor: | Maciej S. Lesniak, Peng Zhang, Megan E. Muroski, Haley Moore, Alan L. Chang, Aida Rashidi, Yu Han, Aurora Lopez-Rosas, Jason Miska |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
T cell Science Carbohydrate metabolism Biology Article 03 medical and health sciences Mice 0302 clinical medicine T-Lymphocyte Subsets Quantum Dots medicine Tumor Microenvironment Animals Fluorescent Dyes chemistry.chemical_classification Tumor microenvironment Multidisciplinary Staining and Labeling Effector Fatty Acids Fatty acid FOXP3 Metabolism Flow Cytometry 030104 developmental biology medicine.anatomical_structure Glucose chemistry Biochemistry Medicine 030215 immunology Conjugate |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-11 (2017) |
| ISSN: | 2045-2322 |
| Popis: | Fatty acid (FA) metabolism directly influences the functional capabilities of T cells in tumor microenvironments. Thus, developing tools to interrogate FA-uptake by T cell subsets is important for understanding tumor immunosuppression. Herein, we have generated a novel FA-Qdot 605 dye conjugate with superior sensitivity and flexibility to any of the previously commercially available alternatives. For the first time, we demonstrate that this nanoparticle can be used as a specific measure of fatty acid uptake by T cells both in-vitro and in-vivo. Flow cytometric analysis shows that both the location and activation status of T cells determines their FA uptake. Additionally, CD4+ Foxp3+ regulatory T cells (Tregs) uptake FA at a higher rate than effector T cell subsets, supporting the role of FA metabolism for Treg function. Furthermore, we are able to simultaneously detect glucose and fatty acid uptake directly within the tumor microenvironment. Cumulatively, our results suggest that this novel fluorescent probe is a powerful tool to understand FA utilization within the tumor, thereby providing an unprecedented opportunity to study T cell FA metabolism in-vivo. |
| Databáze: | OpenAIRE |
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