Detoxified Endotoxin Vaccine (J5dLPS/OMP) Protects Mice Against Lethal Respiratory Challenge with Francisella tularensis SchuS4
| Autor: | Nicholas A. Parejo, Wilbur H. Chen, Stephanie Mott, John E. Palardy, Christine A. Anderson, Alan S. Cross, Sara J. Heninger, Steven M. Opal, Andrew W. Artenstein, Stephen H. Gregory |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Lipopolysaccharides
Serum Population Colony Count Microbial chemical and pharmacologic phenomena Neisseria meningitidis Biology medicine.disease_cause Article Microbiology Tularemia Mice Bacterial Proteins medicine Animals Francisella tularensis education Lung Mice Inbred BALB C education.field_of_study Attenuated vaccine General Veterinary General Immunology and Microbiology Public Health Environmental and Occupational Health medicine.disease biology.organism_classification Antibodies Bacterial Survival Analysis Virology Immunoglobulin A Endotoxins Vaccination Bacterial vaccine Infectious Diseases Liver Immunoglobulin G Bacterial Vaccines bacteria Molecular Medicine Female Bacterial outer membrane Bronchoalveolar Lavage Fluid |
| Zdroj: | Vaccine. 28:2908-2915 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/j.vaccine.2010.01.067 |
| Popis: | Francisella tularensis is a category A select agent. J5dLPS/OMP is a novel vaccine construct consisting of detoxified, O-polysaccharide side chain-deficient, lipopolysaccharide non-covalently complexed with the outer membrane protein of N. meningitidis group B. Immunization elicits high-titer polyclonal antibodies specific for the highly-conserved epitopes expressed within the glycolipid core that constitutes gram-negative bacteria (e.g., F. tularensis). Mice immunized intranasally with J5dLPS/OMP exhibited protective immunity to intratracheal challenge with the live vaccine strain, as well as the highly-virulent SchuS4 strain, of F. tularensis. The efficacy of J5dLPS/OMP vaccine suggests its potential utility in immunizing the general population against several different gram-negative select agents concurrently. |
| Databáze: | OpenAIRE |
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