Genome-wide analysis of KAP1, the 7SK snRNP complex, and RNA polymerase II
| Autor: | Carlos Guzman, Ryan P. McNamara, Jonathan E. Reeder, Iván D'Orso |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Larp7 La-related protein 7 TRIM28 lcsh:QH426-470 Cdk9 cyclin-dependent kinase 9 CycT1 cyclin T1 RNA polymerase II Biology Biochemistry Transcription elongation 03 medical and health sciences 0302 clinical medicine 7SK RNA Data in Brief P-TEFb/7SK snRNP Genetics Transcriptional regulation Bp base pairs snRNP KEC KAP1-7SK snRNP early elongation complex KAP1 Kruppel-associated protein Pol II RNA polymerase II Kinase snRNP small nuclear ribonucleoprotein MePCE methyl phosphate capping enzyme Promoter Hexim1 hexamethylene bis-acetamide inducible protein 1 Cell biology ChIP chromatin immunoprecipitation ChIP-seq GRO-seq Global run on sequencing lcsh:Genetics 030104 developmental biology KAP1 biology.protein TSS transcription start site Molecular Medicine qPCR quantitative PCR Transcription factor II D ChIP-seq chromatin immunoprecipitation sequencing 030217 neurology & neurosurgery P-TEFb positive transcription elongation factor b Biotechnology |
| Zdroj: | Genomics Data, Vol 7, Iss, Pp 250-255 (2016) Genomics Data |
| ISSN: | 2213-5960 |
| DOI: | 10.1016/j.gdata.2016.01.019 |
| Popis: | The transition of RNA polymerase II (Pol II) from transcription initiation into productive elongation in eukaryotic cells is regulated by the P-TEFb kinase, which phosphorylates the C-terminal domain of paused Pol II at promoter-proximal regions. Our recent study found that P-TEFb (in an inhibited state bound to the 7SK snRNP complex) interacts with the KAP1/TRIM28 transcriptional regulator, and that KAP1 and the 7SK snRNP co-occupy most gene promoters containing paused Pol II. Here we provide a detailed experimental description and analysis of the ChIP-seq datasets that have been deposited into Gene Expression Omnibus (GEO): GS72622, so that independent groups can replicate and expand upon these findings. We propose these datasets would provide valuable information for researchers studying mechanisms of transcriptional regulation including Pol II pausing and pause release. Keywords: P-TEFb/7SK snRNP, KAP1, RNA polymerase II, ChIP-seq, Transcription elongation |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|